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Phage display library selection of a hypoxia-binding scFv antibody for liver cancer metabolic marker discovery

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Academic year: 2020

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Figure

Figure 1: Panning procedure of the human scFv Ab phage display library. For each round of panning of the rescued phage library, sequential selections were carried out: positive selections (+) were performed on hypoxic treated HCCLM3 cells at the first and
Figure 2: Panning and screening of hypoxia-binding scFv Abs. ((Left) cells was determined by phage titer assay
Figure 3: Characterization of the hypoxia-binding H103 scFv Ab. (staining with secondary anti-His and tertiary AF647 conjugate
Figure 4: Internalization and binding analysis of the H103 scFv Ab. (incubated with H103 scFv Ab either in phage-display form or soluble form
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