Oral abstracts

(1)

Oral abstracts

O1.1

#

stringent response pathway, facilitated by genomic diversity

Yuan

Li

1

,

Nicholas J.

Croucher

1

,

Claudette M.

Thompson

1

,

Krzysztof

Trzcinski

2

,

William P.

Hanage

1

,

Marc

Lipsitch

1

1_{+ ' (&%+%?>+\\)\>)\}2_{? \ \&! (}

( (( '' ( ( \ H((' H(%+ ] (' ¢¢ ( P ('( %) ' >}="$ 6](&F &F/¦$]( (((] resistance to non-opsonic neutrophil-mediated killing in vitro, a phenotype correlated with in vivo % X P '( # ( >!$ >}=" ' %&'' \] (in silico ¢¢ '( P( (\ 6(( $ ( .%&(]( (H((( (( (]' >¬¢#$N>&>+$''( % & \ ( >¬¢# ] ]( ] &F/¦(>}="%&](6>¬¢# ( .(' % \' >+&F>¬"¦$]( .%

O1.2

Genomics reveals the worldwide distribution of multidrug-resistant

serotype 6E pneumococci

Andries

van Tonder

1

,

James

Bray

1

,

Lucy

Roalfe

2

,

Rebecca

White

2

,

Marta

Zancoli

2

,

Sigríður

Quirk

3

,

Gunnstein

Haraldsson

3

,

Keith

Jolley

1

,

Martin

Maiden

1

,

Stephen

Bentley

5

,

Ásgeir

Haraldsson

3, 4

,

Helga

Erlendsdóttir

3, 4

,

Karl

Kristinsson

3, 4

,

David

Goldblatt

2

,

Angela

Brueggemann

1

1_{' H (\H (\\}2_{' ?=(\=(\\}3_{' F(\.Q'.\\}4_{=(' +\}

.Q'.\\5_{X& > F\+H\}

(2)

O1.3

The post-vaccine microevolution of invasive pneumococcal disease

Fredrick M.

Mobegi

1

,

Amelieke J.H.

Cremers

1

,

Marien I. de Jonge

1

,

Sacha A.F.T.

van Hijum

1

,

Jacques F.

Meis

3

,

Peter

%

W.M

Hermans

1

,

Gerben

Ferwerda

1

,

Stephen D.

Bentley

2

,

Aldert

Zomer

1

1_{((' (? \!Q\&! (£}2_{X& > F\? (\£}3_{Canisius-Wilhelmina Hospital,}

!Q\&! (

)B (( '>\ ''(F_$ ( (% +]' \ . ( ( (( ( % X '( ] ' <( F_ ! (% We serotyped and sequenced 350 strains of Streptococcus pneumoniae isolated between 2001 and 2011 from adult F_ !Q% )B \ \ ( $ \ ] (% ) ((( * '( %? ]] ( \((' ] ( ( ( ] % ( 6' Q('?z$""¢ (( ' ( ] '(\ ] ( %&(' ( ( B (?zp¶"%"""$% ( 6H(' B ] (] ?z\]( ( ' %_ (B ( ']?z\] '( . (' \] 6H(( ( %& '] ( \ (F_%X ]P '((' Q (\' '(F_%

O1.4

Mechanisms of pneumococcal evolution over short and long timescales

Nicholas

Croucher

1

,

Paul

Coupland

2

,

Abbie

Stevenson

3

,

Allanna

Callendrello

3

,

Stephen

Bentley

2

,

William

Hanage

3

1_{F ?\=(\£}2_{X& > F\? (\£}3_{+ ' (>+\\>)}

(3)

O1.5

Genetic stabilisation of the pandemic and drug resistant PMEN1

###

system

Rory

Eutsey

2

,

Evan

Powell

2

,

Janina

Dordel

3

,

Tyson

Clark

4

,

Jonas

Korlach

4

,

Garth

Ehrlich

5

,

N. Luisa

Hiller

1, 2

1_{? ' \ \)\>)£}2_{)+!] .\ \)\>)£}3_{&X& > F\? (\}

£4_{@\ .\?)\>)£}5_{_ H' ?(\>)}

The human pathogen Streptococcus pneumoniae $ H ( (' ( %F] (( ' %&!(\6( %+ H]! (6!( \]H' !(( !% J(@J$ Y \ ( _F _FFJ( % ' '(!( _FFF\ (6 _%? _FFF( (('(( ((_!) P #*6)&?6*\ ( ?6# 6@ _!) % X ] _FFF ( P ( in vitro (\ ] ( ] ( _!) ' ( _!)% \ ] ' (@( ] !] _FFF(( (\(] .? ( '(( ! %X JQ ( P£ (J' H %

O2.1

MapZ beacons the division sites and positions FtsZ

-

rings in

Streptococcus pneumoniae

Aurore

Fleurie

1

,

Christian

Lesterlin

2

,

Sylvie

Manuse

1

,

Chao

Zhao

1

,

Caroline

Cluzel

1

,

Jean-Pierre

Lavergne

1

,

Mirita

Franz-Wachtel

3

,

Boris

Macek

3

,

Christophe

Combet

1

,

Erkin

Kuru

4

,

Michael

Van-Nieuwenhze

4

,

Yves

Brun

4

,

David

Sherratt

2

,

Christophe

Grangeasse

1

1_{?!>6' =\=\ £}2_{' H (\H (\£}3_{' &\&\ £}4_{F(' \}

\>)

(4)

Competence for genetic transformation in

:

primary sigma factor mutations enhance transcription of late genes in

comW

mutants

Yanina

Tovpeko

,

Donald A.

Morrison

\?\(_'\_ >\' F?\?\F\>)

Streptococcus pneumoniae \ '

( (% > (( ' ' factor ½_{\ (('( %½}_{recognises a non-canonical promoter upstream of late competence} \( P ( _!).%+]' \ P (6@ protein, ComW, regulated by the same pheromone circuit as ½_%X(((_comW "6 \]rpoD, encoding the primary sigma, ½A_%½A*_{mutants transformed at an elevated rate} "X&$\ 'comW "%"$%½A*_{proteins carried altered residues in RpoD region, 4 that}

( ½A_{] ¯%&}_comW_{!)\] (}

H %=H ] ("¥" WT levels in the ½A*_{\ ("}_comW_{mutant, consistent with the idea that ComW}

increases ½_{!)\(] %X½}_']

(¥"X&'½A*_{\ ("X&'\} ((( ?X('( %

> (!> %?6""¥¢$

O2.3

A new role for Autoinducer-2 in

Claudia

Trappetti

1

,

Bart

Eijkelkamp

1

,

Zarina

Amin

1

,

Adrienne

Paton

1

,

Christopher

McDevitt

1

,

Marco

Oggioni

2

,

James

Paton

1

1_{' )((\)((\) £}2_{' = \= \}

'' ' (' H \ ('( % ? ] ( '( ( ( ( ( % & (\ ( 6' ( 6' )( 6 )F6$% )F6 ( Y =H> >J J $ 6 ( ' >J J % + =H> ' ( \ . %&( ( (Streptococcus pneumoniae, AI-2 accelerates and modulates ( % F (\ ] ( ( ] .] (( ' luxS

' ](6 _¦\ ( ( ( @( )F6 (% Mice were challenged intranasally with either wild-type or luxS ()F6]( (Y ( 6%& ])F6@ (' (' mice challenged with the luxS% ' \ '']( ((('

luxS( ('](6 %& '] )F6(

( \] (!)6. %> \]( . ] ( H ( ] ( ] ](6 luxS

\ ( H ' . ( ( )F6 ( (% & ( '(

'()F6 S. pneumoniae( ( ((%

(5)

O2.4

Spatial and dynamic organisation of the chromosome and DNA

replication machinery in

Morten

Kjos

,

Renske

van Raaphorst

,

Jan-Willem

Veening

' \ \&! (

? '6(Streptococcus pneumoniae cells has been shown to depend on other .] ' (6(( %> \ ( ((( %X (] ((S. pneumoniae and how this process is connected _!) ( ('% X ' ( ] ' ( ( %& ($\< 6 >$£($\ 6parS

( %'P]] ](''

S. pneumoniae 6$ < % \

< ( ] ((]

((]_!) %X]S. pneumoniae ( ]

((( (]%& B _!) ('P %] \ B( '(\ ( % & (6 @( ( (% + ] (\(6 %]\]] '(>?H' %& \ our data suggest that S. pneumoniae_!) ( ( ' %

O2.5

\ <\\>

redox sensor in

David

Wright

1

,

Andrew

Maule

2

,

Lanlan

Han

3

,

Nicholas

Silvaggi

3

,

Nicholas

Croucher

1

,

Stephen

Bentley

4, 5

,

Thomas

Clarke

1

,

Andrew

Ulijasz

1

1_{F ?=(\=(\£}2_{' X\(\X\>)£}3_{' X\].\X\>)£}

4_{X& > F\+H\£}5_{' ? (\? (\}

(H. '' ] %& B. ( (_!)((!) ' H( ( 'H>$%_ (\Streptococcus pneumoniae must sense and '6 ((>\]](] P( H( ( 6 (% ]](

(.] (H6 e.g. (H$%+ ](

(6)

Cpl-711, a powerful enzyme against pneumococci

Roberto

Díez-Martínez

1, 2

,

Héctor D.

de Paz

1, 6

,

Esther García

Fernández

1

,

Noemí

Bustamante

4, 3

,

Chad W.

Euler

2, 5

,

Vincent A.

Fischetti

2

,

Margarita

Menéndez

4, 3

,

Pedro

García

1, 3

1_{_ ( { {(F\? (F'\?>F?\( (\>£}2_{Laboratory of}

(F\&. ' \!]} .\>)£3_{?F( (( ?F>$\( (\>£}

4_{_ (U{6{\FU{6{\?>F?\( (\>£}5_{Department of Medical Laboratory Sciences,}

+ ?\?!}\!]} .\>)£¢_{F_ \_ \+>[(_\}

-lona, Spain

& 6 ( 6(( (

' Streptococcus pneumoniae%?6(?6 Y((

£?6( (6]\] ?6 ( % F ( ( ?6 ' ?6>$\ ] ] ' \ (' Y%& ]Y] '( ('\] '((' Y (\. (](($ ?6(?6>Y%&Y'' proteins was analysed in vitro and validated in vivo (%?6\Y ( ?6>(. (](( ?6\ '(. Y\(6 %>@\#ÆN?6.( º%#¢ \( ((@ %Q( ] _¦¬F ] (]Q?6( (¢" \]#" ]?6%)] Y (\?6]] ( . % & '( Y ' 6 %

O3.2

Esters of bicyclic amines: a new generation of antimicrobials against

pneumococcus

M. de Gracia

Retamosa

1

,

Roberto

Diez-Martinez

2

,

Beatriz

Maestro

1

,

Esther

Garcia-Fernandez

2

,

Bas

de Waal

3

,

E. W.

Meijer

3

,

Pedro

Garcia

2

,

Jesus

Sanz

1

1_{' + (Y\\>£}2_{?Q> (F'?@\( (\>£}3_{('' &\}

('\&! (

X( '] )$\ ( \

]in vitro38%&(']

( ] ( 6( ?$% +]' \ $ (( (\ ' %& ' ( (\ ] ' ]( ( % \ ] ( ' ?6 (?6 H38 ( ¥)( ''] ( < ( ] 6( ( =) (?6=) $%( ] (((in vitro cultures, monitoring ]\ ( '% & ' ) ( '' (( ( ]( % (\]'((( g2-dendropine$¥ ] ( %) '\g2-dendropine ¡#\"""6( ( \(]? %&\]'(@(((] ()$ 'in vitro against S. pneumoniae (\( ( ( ' ( (( %

% , YY ), { , Sanz JM% F 6( ( ]

(7)

% >'6 | !, Retamosa MG, , >, Rodes MJ, { , Sanz JM, Hermoso JA% ? ?

H(] ( ' (' Streptococcus pneumoniae%

%%)"£¥"\¦6#

O3.3

#

Streptococcus

pneumoniae

using Tn-Seq

Andrew

Fenton

,

Thomas

Bernhardt

,

David

Rudner

+ ' ((>\\\>)

& ] '(( Streptococcus pneumoniae% The main enzymes that build the cell wall are synthases belonging to the family of factors called penicillin-binding $\] ( (6( %6 the rise in S. pneumoniae\(' ] % ' '] ((]].]]H( ('' %F(@' ] P ( ( ]\] (] (%F \ ( ''(( ((\ .]]Y (

in vivo%&(( \]'(&6>P( ]Q

S. pneumoniae% ' (' ]

] P ( ('%&(' ( ']((] ( (' %

O3.4

Engineered liposomes sequester pneumolysin and protect from severe

invasive pneumococcal disease in mice

Daniel

Neill

1

,

Suzanna

Gore

1

,

Laura Bricio

Moreno

1

,

Eduard

Babiychuk

2

,

Aras

Kadioglu

1

1_{' =' \=' \\}2_{' \ \>]Y (}

(8)

DNA release by

autolysin LytA induced

Krueppel-like factor 4 expression controlling pneumococci-related

innate immune response in macrophages

Janine

Zahlten

1

,

Toni

Herta

1

,

Christin

Kabus

1

,

Jan-Moritz

Doehn

1

,

Pedro

García

2, 3

,

Norbert

Sut-torp

1

,

Stefan

Hippenstiel

1

1_{_ F (NF_( (\? `\' ²(Y \ \ £}2

Departa-( { \? Departa-(F'\?Q> Departa-(F'?|@\Departa-( Departa-(\>£3_?F(

Enfermedades Respiratorias, Madrid, Spain

F(]'(Streptococcus pneumoniae6((=( ]( '( $% > S. pneumoniae6=) (( = H \ H S.

pneumoniae_!) (=(\(((_!).(=(\]( (S. pneumoniae

_!) =H %H &=¦6N6(_¥¥6N6 '( ] ''(6 (=H %> with S. pneumoniae6=)(_!) \]]. $ $_!)\((=H %'_!) N( )>?6N6(>&F!6N6$ did not reduce the S. pneumoniae6 (=H %)'(S. pneumoniae\ \ ( '( ( ' ¿((¿ (=%& (( = H ¿ (( &=¦¿(( .] _!) % > _!) ]¡(=H \]( ((( ' $%&'= ]( in vivoHH$(( &H6? ¤N.HNH % & = ((S. pneumoniae6(( ? ( ( F=6" % & =H ( 6< ( ( %

O4.2

^

pneumococcal disease in the ears and lungs of co-infected infant mice

Jayne

Manning

1, 2

,

Eileen

Dunne

1

,

Kim

Mulholland

1, 3

,

Roy

Robins-Browne

1, 2

,

Richard

Malley

4

,

Odilia

Wijburg

2

,

Catherine

Satzke

1, 2

1_{(?( F\ .'\) £}2_{' \ .'\) £}3_{London School of Hygiene and Tropical}

(\=(\£4_{?( *+\\>)}

(9)

'< (%

O4.4

The effect of macrophage polarisation and delayed apoptosis in the

innate immune response to

infection

Lucy

Morris

,

Helen

Marriott

,

David

Dockrell

' >¡(\>¡(\

>B (]?_% ( ]( with Streptococcus pneumoniae\?_H \ %& (( ' ?_ ( ] ( H 6 6%X ' 6H 6?_6¢¥6 $]( ( (((% Q' ] ( ( ](6 ( ?_¢¥ 6 _£ H \ .( (]((?_¢¥6_(_B S. pneumoniae£ and understand how cytokine and chemokine repertoire changes in response to S. pneumoniae ( ](6 ( ?_¢¥ 6 _ ( _% ] ( '( _$ 66 (](6 ] ( ].£F=6$\F=6"$ F!®¤=>$( ( (] \=F>)(&6?% ( ] (]S. pneumoniae

(@( ( ( '' (% ! .6( ' ( ] P@( ] _)F% > ] ( ( ( . H =F>)%?'(((_!)] (&&(F\ '% ( ( ' (( _!) ( ( ( ' (

% ) S. pneumoniae ( '' ' J ](

( ](6( %& ] (

B S. pneumoniae ¢ ] (% ( < .

_B S. pneumoniae\]' ] (_%

O4.5

Murine respiratory tract microbiome: important interactions with IL-17

and pneumococcal colonisation

Neil

Ritchie

,

Tom

Evans

' ]\]\

F=6.] ]Streptococcus pneumoniae%& ' ](' £F=6 (@(% X ( ]( ( F=6) ( B ( ]S. pneumoniae% ] ( ] # H "5_{cfu of serotype 3}_{S. pneumoniae}_% ! ( ' ' ] . (S. pneumoniae P@(% _!) ] H ( ( z₁-V₂ region ¢> !) ] @( (( % >P ] ( F 6>P Ä % \S. pneumoniae '( ( F=6) % F=6) (] ( ]]%& F=6)]@ ( ](6\]( ((' ( (( %& ](

' (@ S. pneumoniae)\(' @ ]@

] F=6) % & ] ] ( ] (' ( ]F=6) ( ](6 ] % ! ]S. pneumoniae was associated with a marked (' ](6(' F=6)%&(' (' mice that did not have a high abundance of S. pneumoniae% P@ ( (

F=)(]( ' ]Ochrobactrum

anthropi\ \ 6 %F=6

(10)

Zinc homeostasis in

during disease

Bart

Eijkelkamp

,

Charles

Plumptre

,

Jacqueline

Morey

,

Stephanie

Begg

,

James

Paton

,

Christopher

McDevitt

' )((\)((\>)\)

)PYStreptococcus pneumoniae (' %.S. pneumoniae

' )&6( )(?\ ( ] Y6( \ )() ( )()FF% X ' previously shown that in vivo\)()()()FF ( ¡ % X ' (@( )()FF ( ( $ (Y %)Y ( \ ( Y ( ( \ ] ( % & \ Y ÃH ?Y_ ¡ ( Y ( Y %+ ]]((Y% . ( P (% (]Y '( (( (% ( H Y \

Y '((\]] P %X

H (( (Y Y6(@(%_( '(] (( '(]P( %

O5.2

The pneumococcal Mga

Spn

virulence transcriptional regulator

Virtu

Solano-Collado

1

,

Rudi

Lurz

2

,

Manuel

Espinosa

1

,

Alicia

Bravo

1

1_{? (F'\?Q> (F'?|@\( (\>£}2_{H6.6F . .\}

\

F 6' \ ' H B ( 6((.%& ()H) from Streptococcus pyogenes and Bacillus anthracis\ '%&' ( '/ ( %&Spn protein from S. pneumoniae is likely N)H) %F@ (

(' (%X'( (Spn( '

% F ' H 6 P1623B % & '

P PB' $( %X' (

fusions based on the P1623B ( < gfp$ %&

'Spn] ] %

( Spn \]'(@(]Spn(F(FF$%>F located within the PB' \] FF' mgaSpn%&'] P(( ' %( \Spn is able to spread (Q_!) 6_!)H\+6!>(6 ( % _!) contribute to the capacity of MgaSpn H ]( %

(11)

O5.3

Genomic analyses of pneumococci reveal a wide diversity of

bacteriocins, including pneumocyclicin—a novel circular bacteriocin

Carlijn

Bogaardt

,

Andries

van Tonder

,

Angela

Brueggemann

' H (\H (\

( ( H\ ] ( % ( ( $. ( 6( %( (( H( ( ] ' ( (

H%&(] /$ '((( ( blp

( (' ( ( £ $ ' (' H £($'

blp' %X(¢(' ( ¦¢] (\

(@( \(( (P(' \('((

H %X((blp

(](@(' 'blp(%&É N *blp

]' /(¢ (¥'\]

(%](( ((blp] (@(% \

6 ( ( ( ' ] ( \ ] ( ]6 ( % ] ( ( Q H%&(( '('(](

variety of blp \ HH%

O5.4

Antibiotic-induced bacteriocin expression—regulatory interplay

between the

blp

and

com

systems in

Morten

Kjos

1

,

Eric

Miller

2, 3

,

Frank

Lake

1

,

Daniel E.

Rozen

2

,

Jan-Willem

Veening

1

1_{' \ \&! (£}2_{=(' \=(\&! (£}3_{&' \ \}

H H ((Streptococcus pneumoniae% !)6P\ ] ( H 6( ( ubiquitous bacteriocin blp gene-cluster in S. pneumoniae_¦%& blp6 ]#

( _blpS blpSRH$\_blpA)?6 ((

(?$\ ($( .]_blpT(_{>_¬""¢}$%

blp6 ' (P '?%F ( ( (

] blp expression, we made constructs in which all 5 blp ] (' luc$\gfp and lacZ%6H ](] _blpS ] '\ H blp6 ] (( H B (\ ( ( % F( blp-expression was also pH-dependent and strictly dependent on the presence of the blpSRHC %>blp-expression and competence are induced \ +6((\( ( P \](( ] ] % H H ( ?>$ ( (

£(((?>\blp6 ] ]. (\(B (

]((%> Q S. pneumoniae \'H ?\blpAB is not intact _¦ \ ()? ?>\comAB%> .\]]

blp6' P )\H] blp6((((((

(12)

Conserved

spirosomes point toward a

single type of transformation pilus in competence

Petya V.

Krasteva

1, 2

,

Raphael

Laurenceau

1, 2

,

Amy

Diallo

1, 2

,

Sahra

Ouarti

1, 2

,

Magalie

Duchateau

1, 2

,

Christian

Malosse

1, 2

,

Julia

Chamot-Rooke

1, 2

,

Remi

Fronzes

1, 2

1_{F \ \ £}2_{?!>#¥\ \}

The success of Streptococcus pneumoniaeQ ( .\ ]¡ ( %! \ ' .( H_!)( ( (\ Y (.' %_!). P H .( ( ( ( ' (% X previously reported a long, Type 4 pilus-like appendage on the surface of competent pneumococci that likely binds H _!) \] ( ( ( \É(* (( _!)]%+ ]]É(* 6 @ ( %X ( Y((6(( ¿ ¿ ( '( .(%

O6.1

Pathogenesis of non-encapsulated

in

experimental otitis media

Larry

McDaniel

' (? \[.\>\>)

( $ ( ' ( %Streptococcus

pneumoniae $ % & (' (

' ( (% X Q ' ?z$\ ' ( ( ( (

S. pneumoniae !>$% X ' ' ( ( $

!>( ' %)((!>'(@(]

cps' (()?()_%( (]!>

(13)

O6.2

Modulation of nasopharyngeal innate defences by viral co-infection

predisposes individuals to experimental pneumococcal carriage

Sarah

Glennie

1, 6

,

Jenna F

Gritzfeld

1

,

Shaun H

Pennington

1

,

Michael

Garner-Jones

1

,

Nicholas

Coombes

1

,

Mark J

Hopkins

2

,

Cintia F

Vadesilho

3

,

Eliane N

Miyaji

3

,

Duolao

Wang

4

,

Angela D

Wright

5

,

Andrea M

Collins

5

,

Stephen B

Gordon

1

,

Daniela M

Ferreira

1

1_{F \=' >& (\=' \£}2_{=' >z ? \=' (}

' +\=' \£3_{? (\F\>\ Y£}4_{& ?& \=' >}

& (\=' \£5_{!F+=' ( ( ' +!+>& \=' \£}¢_{School of Cellular and}

(\' \ \

>( Q ( (<Y%F ( ( ( ] % X ( H ('] ' (('( %( ( ( Q ] ( ' % z ] ( ] ( .( # ' ' ( ' ¢ ' ' subjects; p·"%"$%! ++$'] (' 'Q(] (] (

(%in vitro epithelial model we explored the impact of increased mucosal FH in the context of

6%<(+( ( (( % ( ] .( ( +6( ? ?$% ? '(('(.((+( %X +(

?in vivo.(+N( '

( 6?(%X ?6('.(+( ( ]( ' %X]]'' (<Y' ( (] ''(%

Acknowledgments:(((\!F + !F+$=? '

!] .\(( ?N> ()>$%

O6.3

Circulating pneumolysin is a potent inducer of cardiac injury during

pneumococcal infection

Daniel

Neill

,

Yasir

Alhamdi

,

Simon

Abrams

,

Hesham

Malak

,

Richard

Barrett-Jolley

,

Gouzheng

Wang

,

Cheng-Hock

Toh

,

Aras

Kadioglu

' =' \=' \

Streptococcus pneumoniae accounts for more deaths worldwide than any other single pathogen through diverse disease

(14)

.( %&]('](] (((H ( (( ( ( ] ( % ( (( H6(( (Q (Q =} (( %

O6.4

Streptococcus pneumoniae

invades the heart during severe pneumonia

in a non-human primate model

Luis

Reyes

1, 4

,

Nilam

Soni

1, 2

,

Cecilia

Hinojosa

1

,

Jessica

Perry

4

,

Robert

Shade

4

,

Melissa

de la Garza

4

,

Luis

Giavedoni

4

,

Marcos

Restrepo

1, 2

,

Carlos

Orihuela

1

1_{&' &H+>? >)\>)\&H\>)£}2_{South Texas Veteran Health Care System, San Antonio,}

&H\>)£3_{' (((>\\?£}4_{>]! ? \&H( F\>}

)\&H\>)

) H"(( H (' ('%X'

( 'Streptococcus pneumoniae translocates into the hearts of mice during experimental

''(£]](' ( ( %&H ] ( %& Q]( whether S. pneumoniae'( 6 ( %J6 6(n

· $ ] ( ] ( \ \ \ ( ( % )(] ( ]S. pneumoniae &F%6%#H"8_{?$%_} ]6(((H\P ((\( .'% ] @(]('( ( B "(% ( (]((] %? ((]( H (%&]('(](6 (' \ (( %F] (.](' .B (% ('( ' ( ( ( % ?. ]( ' < (' %? ( \ ('] (\ ] '( ]%( ] ((] (% @ ? ( 6 ( ] '(% _ 6 \ '( % & H (( (( (]' (]' (%&@( ' %

O6.5

#

Jimstan

Periselneris

,

Tracey

Pollard

,

Thomas

James

,

Mahdad

Noursadeghi

,

Jeremy

Brown

' ?=(\=(\

Streptococcus pneumoniae ]' '<

( %]6 (' S. pneumoniae

(15)

( ](6((@&F\ < ( _. %F(\ 6<

(&!(F=¢ ' (@ ] (S.

pneumoniae ] _% F ( ( (

)= ( ( ](6 &F ( \ )= . ] ( %&((H( < \]((%& <( ( ] S. pneumoniae virulence, ] ( ( %

O6.6

A phase-variable genetic switch regulates pneumococcal virulence via

epigenetic changes

Ana

Manso

1

,

Chai

Melissa

3

,

John

Atack

4

,

Leonardo

Furi

1, 2

,

Megan De Ste

Croix

1

,

Richard

Haigh

1

,

Claudia

Trappetti

3

,

David

Ogunniyi

3

,

Lucy

Shewell

4

,

Mathew

Boitano

5

,

Tyson

Calrke

5

,

Jonas

Ko-rlach

5

,

Matt

Blades

1

,

Evgeny

Mirkes

1

,

Alexander

Gorban

1

,

James

Paton

3

,

Mike

Jennings

4

,

Marco

Oggioni

1, 2

1_{' = \= \£}2_{' (>\>\F£}3_{' )((\)((\) £}4_{¡' \> \}

Australia; 5_{@\ .\>)}

>]] É*$' ''( (\ (' (((% + \]]( ' &F 6(@$>_¦FFF%& (@ ¢( Y]¢ '@(( \(@( 6 6%X ( ]H >_¦FFF ' ('] ](H @%X( (' H .( ( X& ( (@( in vivo ]]>_¦FFF' %>_¦FFFP\( % F' @ ( ( ] ( @ % (]' ] ] (\( (in vitro and in vivo%

O7.1

Pneumococcal conjugate vaccine reduces the rate, density, and

( _

human challenge testing of a pneumococcal vaccine—a double blind

randomised controlled trial

Jenna

Gritzfeld

1

,

Andrea

Collins

1, 2

,

Angela

Wright

1, 3

,

Elena

Mitsi

1

,

Carole

Hancock

2

,

Shaun

Pen-nington

1

,

Duolao

Wang

1

,

Ben

Morton

1

,

Daniela

Ferreira

1

,

Stephen

Gordon

1

1_{=' >& (\=' \£}2_{=' ( ( ' +& \=' \£}3_Local

? ' !] .\=' \

!]' (( ' %>(' ¡ ' ( ¡ ( 6' ( (] ] ' ((% X ' ('( ( ( H +?$ (£ ] 6' Q ' ?z$ ' ®_on

(16)

" 3#N¥8$ ( ¥ 3N¥8$ 3. "% 3?F "%"¦J"%#8£p ¶ "%""$% ( (( ] ((?z ( ]%& ( '(' ($]@ ((?z ( #¦' \¥\p·"%"$%?z (( \(\(( (% &+?((¡(( '¡]' £?z '((( (]''%

O7.2

Heterologous protection against

colonisation by the mucosal adjuvant cholera toxin subunit B

Kirsten

Kuipers

,

Dimitri

Diavatopoulos

,

Corné

van den Kieboom

,

Fred

van Opzeeland

,

Elles

Si-monetti

,

Dorette

van Ingen-Schenau

,

Mariska

Kerstholt

,

Malgorzata

Borczyk

,

Mihai

Netea

,

Marien

de Jonge

((' (? \!Q\&! (

((\ H?&$( .(Q'H ' ('(](((Q'(%(]( '( %X ( '(?& \' (('H %+ \]] ?& ( Streptococcus pneumoniae\ ( ( (%?& (H ?_\F!6®(F=6"\' &(&N& % .\?&( <H&( ( ] ('%&6@ \?& P (6 ( \ ' ] ( ] 6 (% )((\ ]>?F_6N6_{and CCR2}6N6_{\ &(} %> .\?&6(( ( ( (( ( ( \

( ( \ \(( S. pneumoniae %

& .](\] @ ?& ( heterologously protects against S. pneumoniae %In vivo experiments are ongoing to assess the role of <% F in vivo (' ( ?& (''(Q'%

O7.3

In vivo

$

pneumococcal vaccines

Jenny

Herbert

1

,

Emily

Kay

2

,

Jon

Cuccui

2

,

Vanessa

Terra

2

,

Brendan

Wren

2

,

Timothy

Mitchell

1

1_{' \ \£}2_{=(>+(& (\=(\}

. ( ( #\ )F_>\ ( (%& pneumococcus is the leading cause of bacterial pneumonia in children, but can also cause disease in the elderly and (% ' ®_{, a protein polysaccharide conjugate vaccine, is currently used in children to prevent}

'' (% & ' ( '(%=' \''(' ] (% ( ]' %('( ( ''(] (']

38%&(](@(Campylobacter

jejuni ] ' (

(17)

to pneumococcal type 4 polysaccharide to be done in Escherichia coli, ( % &( '] (Q' (% ' ¡ ] (

Q' (' ' ®_{$(%_}

will be presented showing that a glycoconjugate vaccine produced in E.coli ( %

% & z>\_?\}=\)((>\?[\X X%('

(Q'(%[( "£¢/¦¦J¢%

F_/#¢/NN(H%(% N"%"¦¦NQ%"%"¦¥6"

O7.4

Immune responses to pneumococcal vaccination in HIV-infected adults

in the UK

Sian

Faustini

1, 2

,

James

Hodson

2

,

Sindiso

Masuko

2

,

Mebie

Singo

2

,

Joyful

Chigiga

2

,

Jenny

Herbert

3

,

Timothy

Mitchell

3

,

Tim

Plant

1

,

Mark

Drayson

1

,

Kaveh

Manavi

2

,

Calman

MacLennan

1

,

Alex

Richter

1

1_{?F> '\>F(F\' \(\£}2_{HIV Service, Queen Elizabeth Hospital}

\(\£3_{F (F\>F(F\\' \}

(\

Streptococcus pneumoniae Q ( +Fz6 ( ('(% 'H z6$\

('\ (( '+Fz6((+Fz)\""¥$ ¡ ( (%)F $ ]+Fz 'UY+\ (?F> '\ ' \(( (('(]+Fz% &)F(( H#" (z6\( 6(6'F ( \\\#\¢\\¦z\\¥?\¦)\¦\($X+ ' ("%#ÆNº¥N (¦6HH6( ($%)F

]H] ' ®_{?z6$\ (6Q'\(}

( / ¥+Fz6( ( ] '( ] z6 ( ( ] ¦'( ] ?z6%?z6F ] P'z6( ( p ·"%"#$\¦zp·"%"$\¦) p·"%""$(¦p·"%"$\(H(.% ( '( (?z6%](@ '(z6(] (] '?z6] ( ]] ]?z6]'\ ](@( ' ('%+]' \ '( ' ] (?z6%?z6(z ( F +Fz6( ?z6(\.z%& '(@( P @ (( ( %

O7.5

The agglutinating effects of anti-capsular antibody contribute to

pneumococcal clearance

Aoife

Roche

,

Jeffrey

Weiser

' '\>)

)Q @Q' ( (\ ( . P % & ] ( 6 ( ( '% X ' ( (

of Streptococcus pneumoniae( (%X '

(18)

'(6'(] (%F)Q \ '( %X]( ] 6@F)).(F)6 ($\ 6 (](6 ($%X']((<] 6( P(6(( %]( ( ((]6] % \' ] (( P%F((\ ( ( ) ] % & ' (\( (%&( ((( ']'(%+]' \' 6 F(6 (] ' %

O8.1

PBP2b, MreD and DivIVA constitute a functional unit in the peripheral

peptidoglycan synthesis machinery of

Daniel

Straume

,

Kari Helene

Berg

,

Gro

Stamsås

,

Leiv Sigve

Håvarstein

! ]' =>\)\! ]

) ''( (Streptococcus pneumoniae%& '(\ ?\ _\()\_'Fz)\( (( %X' ' ](6( H ( (@ (38%) ] ( ]_'Fz) (((38\(_'Fz)' % ('('(( ( H% '( ] % +]' \ H( ( ] %F ( ] ( ( .( % ( \?_\] ' %& ? ( ( (%X(' (6((((] longer able to protect themselves even though they were ComM¤_{%&] '(} ]_'Fz)( _(((\( \_'Fz)( _ P (?_H ? %_ ?\ (\((<('%&@(( _'Fz)( _ P ( '(N ' %

% +\>)\> _\+' =>%]' ( (> ¢%[ "£¦#/J#%F_/¥¦¢ /NN(H%(% N"%¥N[%""¥6

% )\>\?\?!\?Y?\=' [%F N 6.

>.( _'Fz)((('%=>

(19)

O8.2

Conformational plasticity of choline-binding modules:

-hairpin to

-helix transition of choline-binding repeats triggered by detergent

micelles and membrane vesicles

Hector

Zamora-Carreras

1

,

Beatriz

Maestro

2

,

Erik

Strandberg

3

,

Anne

Ulrich

3

,

Jesus

Sanz

2

,

M.

Angeles

Jimenez

1

1_{?Q> (F'?@\( (\>£}2_{' + (Y\\>£}3_F&\

Karlsruhe, Germany

?6(('¯¯6( (6( ?$\] ¯6 ]( . %&@((?' (' their remaining choline-binding ability, we have analysed the structure of the isolated 238-252 sequence from the LytA

=)_238-252$% ? ( ( ( ( 6 (

'6.¯6 P(( ((% .\ ( P \ °6H Y] (($ ( ((($( \] ('%&¯6 6 6°6H' ' 'H % \66( ( =) ?J=) \ ( ¥¥J¥$\ ( ?\ ( ( ( P %& .](\' ( ( 6(( ]] ( (( \(=)( ?' ' ] ' ]%

O8.3

On the interaction between the pneumococcal cell wall and the

</`.>_

externally added CBMs to bacterial cultures

Manuel

Sanchez

,

Beatriz

Maestro

,

Francisco

Garcia-Asencio

,

Jesus

Sanz

' + (Y\\>

6( ?$.6(6((?$@ ( ] ( ((%? \ \ P(6( ?$%F( '](( ? =)((?=Y?J=)(?J?= '$in vitro inhibits 6]( \ (%&' ] @('6\]@ ((¡?J=) (?J?=_)6( 6 (]%_ ?¢$\( ( (((¡ ( _) % ' \ ]#$ (( ( \ ( \ (( (%X ?] H (((H Streptococcus pneumonia¢\( ]((( \( (( %

&\] ]in vitro(\?(

(20)

Conditional lethal mutants reveal that FtsA is needed at early and late

stages of cell division in

Andrea

Mura

1

,

Daniela

Musu

1

,

Ana Isabel

Rico

2

,

Marcin

Krupka

2

,

Dalia

Denapaite

3

,

Pavel

Branny

4

,

Miguel

Vicente

2

,

Orietta

Massidda

1

1_{' ? \? \F£}2_{? !({\( (\>£}3_{' \ \ £}

4_{)(>?Y\ \?Y}

& (' P \()\(6%+ ] * '&>$\()¦_TS, A20_TS and A21_TS\( 6 (?\ ( ]¥°C but not at 40 µ?%(( () \''( ]

\(N (' %& BH ](\ H_WT,

the A_TS( ]( (B"µ?% 6 ](

that the FtsA_TS proteins lost the ability to self-interact but retained the ability to interact with FtsA_WT ( %

-FtsA_WT\ H ( _Zn inducible promoter, fully complemented the A20_TS and A21_TS phenotype at 40 °C, but )¦_TS%X( (' (H_WT at both temperatures, in the A_TS ] ( B"µ?\((%& results are consistent with what was observed in a zinc dependent *(\]

)]6)H ( _Zn %=(]() P (

¡(6\ ' ' 6'\].)\) P ( %' \ @ )S. pneumoniae and '( ]% ' \( . (' ] ( ('\ ( ] ( ( ( ) (]%

O9.1

Structural basis of PcsB-mediated cell separation in

Streptococcus

pneumoniae

Sergio G.

Bartual

1

,

Daniel

Straume

2

,

Gro Anita

Stamsås

2

,

Inés

3

,

Carlos

Alfonso

4

,

Martín

Martínez-Ripoll

1

,

Leiv Sigve

Håvarstein

2

,

Juan A.

Hermoso

1

1_{_ ? (> \F(U{6{\?>F?\( (\>£}2_{Department of Chemistry,}

((>\! ]' =>\Ås\! ]£3_{? %>}

( \>!? ? ?!F$\( (\>£4_{? (F'?F$\( (\}

Spain

In order to divide, bacteria must synthesise a new septal cell wall, which must be split down the middle by 1 or more ( ( % & 6 X (]¤? 6' %& (\

P ( ] $\] 'Streptococcus

pneumoniae _¦(¢%]' ( 638%&

'(( ] /((]( ] ( ( %& 6 (H' ( (%F \](6((( ' ( ''. (((Y %X (]( ]H(('%%( (('$%F]( ' ( ('\ '( Y (] ( %

% >\> _\>)\~YF\)?\ |Y6%> 6

(21)

O9.2

Single molecule force spectroscopy reveals interaction strength

between

TIGR4 pilus-1 tip protein RrgA and

Tanja

Becke

1, 2

,

Stefan

Ness

1

,

Raimund

Gürster

1

,

Stefanie

Sudhop

2

,

Arndt F.

Schilling

4

,

Anne

Marie

di Guilmi

3

,

Hauke

Clausen-Schaumann

2

,

Markus

Hilleringmann

1

1_{(? \_ )(>( \' )(>\}

Munich, Germany; 2_{? )(& ( '(\' )(>\\ £}

3_{\F(> \?)6?!>6' `[ \ \ £}4_{_ > (}

+(> \.( F \&' ²\\

6'Streptococcus pneumoniae represents a major human pathogen causing serious diseases including

\\( \] ] (](%) ' \ (' ( ( $ ''( ( '% !'S.

pneumoniae&F6( ?] \'.( .

( ) ( % & ) ] ( ] @ .H H?$(%+]' ) ( ( ' ('( ) ( ( ( ((%F \.]@ ((( ] ) (( ( P ( ' ( %F(\] \](( ( ( .\P ] (( ?%F@ ](]@( ] )(@ \] . ]@(] ( ' %X H ( ( )J@ % X ( (( ]6(' ?.@ \\ F\](]%

O9.3

Structural basis for selective recognition of microbial and endogenous

polysaccharides by SIGN-R1 receptor

Iván

Acebrón

1

,

Noella

Silva-Martín

1

,

Sergio G.

Bartual

1

,

Erney

Ramírez-Aportela

2

,

Pablo

Chacón

2

,

Chae Gyu

Park

3, 4

,

Juan A.

Hermoso

1

1_{_ ? (> \F(U{{³´\?>F?\( (\>£}2_{_}

?\F(U{{³´\?>F?\( (\>£3_{= ? (F\? ]}

? F(F_\&. ' \!]} .\!}\>)£4_{= F\>' (}

>F\ => Q (>\}' ?(\>(6\>\ \ Korea

(22)

{ &

potential of pneumococcal teichoic acids

Nicolas

Gisch

1

,

Thomas

Kohler

2

,

Franziska

Waldow

1

,

Dominik

Schwudke

1, 5

,

Holger

Heine

3, 5

,

Regine

Hakenbeck

4

,

Dalia

Denapaite

4

,

Sven

Hammerschmidt

2

1_{_'? \ ? \=Y6? ((\ \ £}2_{_}

\F F (\' ](\ ](\ £3_{Division of Innate}

F\ ? \=Y6? ((\ \ £4_{_ \'}

Kaiserslautern, Kaiserslautern, Germany; 5_{) ] ? ! )?!$\ ? = _=$\}

& .] ( =&) ( X&)$ P 6' %& (\ .H\( (']\] ((( % now, only teichoic acids of Streptococcus pneumoniae have been analysed on the molecular level and the structural (=&) '(%+ \] ( .] .X&)(] '(( from S. oralis%&.](( '(@ ] (( ( ( \] (Y''(&) ( ( % (( =&)\ ( ( (.( Y ( < ( ' (\](6 ( ! % X&) ( ( ( Y(]=)(Y6.Y\@.]'(( ]6(!H % ( '('( $ =? =\?)\( $' &) ( =&)% \]H( &) !?$(((&6. '(( %( \]'&) ('%

O9.5

LocZ is a new cell division protein that directs septum placement in

Nela

Holecková

1

,

Linda

Doubravová

1

,

Orietta

Massidda

2

,

Virginie

Molle

3

,

Karolína

Buriánková

1

,

Oldrich

Benada

1

,

Olga

Kofronová

1

,

Aleš

Ulrych

1

,

Pavel

Branny

1

1_{?( _'\F \'%'%%\)(>?Y\ \?Y£}2

(>Y? \>Y%() \' (? \? \F£3_{Laboratoire de Dynamique des}

F ! P\' `( FFF\?!>\ \

(' ( ( ( ( % +]Streptococcus pneumoniae

(23)

O10.1

interaction with brain-derived neural cells

and alterations in functional state of the cells

Reuven

Afriat

,

Marilous

Shagan

,

Ron

Dagan

,

Esther

Priel

,

Yaffa Mizrachi

Nebenzahl

' !'\ >'\F

Streptococcus pneumoniae]#" ( '' P(

\(\ (\(( %X' '(@(' ' (( ' .] (' %X' ( ''( ] ( ( ''%? ]( @ (( #\!>?6$ ( !( >$ .'( (](6X&$ %F with S. pneumoniae ( & F & F$ '\ @ ( @ H ] ( %&F Y _!) ( H %& ((&F' ( ( \]( ()_ &F)%FY]@ '( ( &F' S. pneumoniae%&\ &F'' ( ()_ )%& ( . ' &F' %

O10.2

PSGL-1 receptor on leukocytes is a critical component of the host

immune response against invasive pneumococcal disease

Elisa

Ramos-Sevillano

1, 3

,

Ana

Urzainqui

4

,

Miriam

Domenech

1, 2

,

Rafael

González-Tajuelo

4

,

Fernando

González-Camacho

3

,

Francisco

Sánchez-Madrid

4

,

Jeremy S

Brown

5

,

Ernesto

García

1, 2

,

José

Yuste

2, 3

1_{? (F'\?>F?\( (\>£}2_{?F>\( (\>£}3_{F(>(? FFF\( (\>£}4_{Hospital de la}

\( (\>£5_{? F<(& %?=\=(\}

. '( Streptococcus pneumoniae. Among \. < ( ((\( (( ¡ ( \ ( . % F ( ] . 6 (6 >=6$ '

]S. pneumoniae( '' %?6(

of S. pneumoniae]>=6]( (\ ' ((']>=6] %

' \ ((Y \(=)\] (@(( (>=6%H ](6((@>=6 ( (>=6. ''(%+ demonstrated that PSGL-1-/-_{'] ' (](6}

(24)

Pneumococcal adhesins PavB and PspC are important for the interplay

with human thrombospondin-1

Ulrike

Binsker

1

,

Thomas P.

Kohler

1

,

Krystin

Krauel

2

,

Sylvia

Kohler

1

,

Hansjörg

Schwertz

2

,

Sven

Hammerschmidt

1

1_{_ \F F (\' ](\ ](\}

-many, 2_{F F(& (\' ( ](\ ](\}

Streptococcus pneumoniae]( ( 6' H

' %&( 6' ( ( 6H( \ ] H H% & (6&>6$ ('(((( 6' ' %+ ]( @ ( (

' '$( ??$ S. pneumoniae

$ ] &>6% & ' ( ? &>6 P ] ( <] (S. pneumoniae_¦«( #)%z H >$ ( =F>) ] H ( ( ' ( ? ] (% \ ( ( S. pneumoniae _¦¸ ( ( (@ '(?&>6 6 (] H(< % &'(?' @(]'(%&(@' ( ? ( &>6 ( ( &>66(( ( %((] '(? ' H &>66(']=F>)(>% \('(? ''('%&((' '(? P &>6 % & ( 6( &>6 (%& (]( ]&>6%

O10.5

Mcl–1 regulates mitochondrial ROS-mediated bacterial killing of

to pulmonary infection in COPD

Martin

Bewley

1

,

Julie

Preston

1

,

Richard

Budd

1, 5

,

Dave

Singh

2

,

Peter

Barnes

3

,

Louise

Donnelly

3

,

Steve

Shapiro

4

,

Moira

Whyte

1

,

David

Dockrell

1, 5

1_{' >¡(\>¡(\£}2_{('\' +> \ \£}3_{!+ (}

== \F ?=(\=(\£4_{' \ \>)£}5_>¡(&+\>¡(\

(25)