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A multiscale framework for microbial evolution to identify the emergence of antibiotic resistance

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Figure 1. Mechanisms of resistance acquisition.
Figure 2. Hierarchy of constraints in evolution.
Figure 3. Evolutionary pathways on the fitness landscapes.
Figure 4. Distribution of fitness effects of new mutations.
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Outline

A deterministic evolutionary model for purifying selection Adaptive selection stochastic evolution model Fitness landscape of TEM-1 for cefotaxime General discussion Conclusions Nicking mutagenesis protocol for TEM-1

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