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Curriculum Vitae. Education: Research Associate (Sept Current)

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Curriculum Vitae Pramod Kumar Giri, Ph.D

Research Associate,

Department of Microbiology & Immunology, Feinberg School of Medicine,

Northwestern University, Chicago, IL 60611 USA. Phone: Lab : 001-312-503-1093

Cell : 001-574-339-7072 E-mail: pramodgiri75@gmail.com

____________________________________________________________________ Education:

Research Associate Department of Microbiology & Immunology, (Sept. 2009- Current) Feinberg School of Medicine,

Northwestern University, Chicago, IL 60611 USA. Postdoctoral Research Associate Eck Center for Global Health,

(Sept. 2006-Oct. 2009) Department of Biological Sciences,

University of Notre Dame, Notre Dame, IN-46556 USA. Ph. D (July, 2006): Tuberculosis Immunology Laboratory,

Department of Biochemistry,

Post Graduate Institute of Medical Education & Research, Chandigarh-160012 India.

M.Sc. (June, 2000): Department of Biotechnology,

Indian Institute of Technology (IIT) Roorkee, Uttarakhand -247667 India.

Ph.D Thesis: Evaluation of new generation Th1 adjuvants for the development of antituberculous mucosal vaccine based on Mycobacterium tuberculosis Ag85 complex proteins.

Ph.D Mentor: Professor G.K. Khuller, Senior Professor (former Head of the Department), Department of Biochemistry, PGIMER, Chandigarh, 160012, India.

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Honors and Awards:

1. Included in Who's Who in America, 2011 Edition. 2. Reviewer for BMC Biotechnology journal.

3. Recipient of Senior Research Fellowship of Indian council of Medical Research (ICMR), Gov. of India, New Delhi [2004-2006].

4. Recipient of Junior Research Fellowship of Indian council of Medical Research (ICMR), Gov. of India, New Delhi [2002-2004].

5. Qualified Junior Research Fellowship of Indian council of Medical Research, Gov. of India (ICMR), New Delhi [2002].

6. Qualified, National Eligibility Test for Junior Research Fellowship of Council of Scientific and Industrial Research (CSIR) Govt. of India, New Delhi. [Dec. 2001].

Research Skills:

• Proteomics techniques like MALDI-TOF, LC-MS-MS, 1D and 2D-PAGE, whole gel electro-elution, multi-elution, Western blotting, N-terminal sequencing. • Protein purification techniques like gel filtration chromatography, ion-exchange

chromatography, affinity chromatography, hydrophobic interaction chromatography etc.

• Handling of pathogenic (M. tuberculosis) and non-pathogenic cultures.

• Tissue culture techniques- Maintaining mammalian cell line, generation of bone marrow derived macrophages/dendritic cells etc.

• Immunological techniques- Generation of hybridoma, generation of bone marrow chimera and adaptive transfer in mice, flow cytometery, ELISPOT, cytotoxic T cell assay, ELISA, lymphocyte proliferation assay, estimation of various cytokines, neutrophil activation assay, macrophage activation assay, western immunoblotting, dot blot, raising antibodies, purification of antibodies. • Cell biology technique- Con-focal microscopy, Phagosome isolation, microvesicles

and nanovesicle (exosomes) isolation etc.

• Molecular biology techniques – DNA & RNA extraction form animal tissue and cells, Q-PCR etc.

• Microbiology techniques – Phagocytosis assay, microbial cultures, colony forming units (CFU) enumeration etc.

• Handling of experimental animals like-Mice and Guinea pigs. Computer skills

1. Flow-cytometry data analysis using Fow-Jo and WIN-MDI software. 2. Statistical software – SPSS and Sigma Plot.

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3. PC applications, MS office, MS DOS, Paint, Adobe Photoshop etc. Publications

1. Choi HJ, Geng Y, Cho H, Li S, Giri PK, Felio K, and Wang CR. Differential requirements for the Ets transcription factor Elf-1 in the development of NKT cells and NK cells. Blood, Dec 2010; doi:10.1182/blood-2010-09-309468 2. Giri PK, Kruh N, Dobos-Elder KM, Schorey JS. Proteomic analysis identifies

highly antigenic proteins in exosomes from M. tuberculosis-infected and culture filtrate protein-treated macrophages.Proteomics. 2010 Sep; 10(17):3190-202. 3. Giri PK, Verma I and Khuller GK. Adjunct immunotherapy with Ag85

complex proteins based subunit vaccine in a murine model of Mycobacterium tuberculosis infection. Immunotherapy 2009 1(1): 31-37.

4. Giri PK. How could we have better vaccines against TB? Expert Opin Biol Ther. 2008 Nov; 8(11):1759-1772.

5. Giri PK, Schorey JS. Exosomes derived from M. bovis BCG infected macrophages activate antigen-specific CD4+ and CD8+ T cells in vitro and in vivo. PLoS ONE. 2008 Jun 18; 3(6):e2461.

6. Giri PK & Khuller GK. Is intranasal vaccination a feasible solution for tuberculosis? Expert Rev Vaccines. 2008 Nov; 7(9):1341-56.

7. Giri PK, Verma I and Khuller GK. Enhanced immunoprotective potential of Mycobacterium tuberculosis Ag85 complex proteins based vaccine against airway Mycobacterium tuberculosis challenge following intranasal administration. FEMS Immunol Med Microbiol. 2006 Jul; 47(2):233-41.

8. Giri PK, Verma I and Khuller GK. Protective efficacy of intranasal vaccination with Mycobacterium bovis BCG against airway Mycobacterium tuberculosis challenge in mice. Journal of Infection. 2006 Nov; 53(5):350-6.

9. Giri PK, Sable SB, Verma I and Khuller GK. Comparative evaluation of intranasal and subcutaneous route of immunization for development of mucosal vaccine against experimental tuberculosis. FEMS Immunol Med Microbiol. 2005 Jul 1; 45(1):87-93.

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Conferences, Symposiums and Professional Training:

1) 2009 (January). Keystone symposia on Tuberculosis: Biology, Pathology and Therapy. Keystone Resort, Keystone, Colorado, USA. Poster Title: Pramod K Giri, Nicole Kruh, Karen M. Dobos-Elder, Jeff Schorey. Proteomics approach to identify the mycobacterial proteins present on exosomes isolated from M. tuberculosis infected macrophages.

2) 2008 (July). 43rd US-Japan tuberculosis & Leprosy Research Conference.. Poster Title: Giri PK, Schorey JS. Exosomes from mycobacterium infected macrophages augment specific T cell immunity.

3) 2008 (May). Oral presentation in Chemistry-Biochemistry-Biology Interface (CBBI) meeting at University of Notre Dame. Giri PK, Schorey JS. Exosomes from M. bovis BCG infected macrophages augment specific CD8+ T cell immunity.

4) 2005 (Feb). Attended International Conference on Functional Genomics for Vaccine & Novel Drug Design against Mycobacteria, at IIT Kharagpur, India [Poster presentation].

5) 2004 (July). Attended 31st Annual conference of Indian Immunology

Society organized by Department of Biotechnology, Anna Malai University, Chennai, India [Poster presentation].

6) 2003 (March). Qualified Advanced Course in Research Methodology and Biostatistics at PGIMER, Chandigarh. This 2-month course was aimed at teaching medical professionals and research fellows the intricacies of medical research viz. study designs and protocols, biases, ethical considerations, subject selection, data collection, processing and analysis. I also learned the use of advanced statistical procedures applied in different research trials and studies.

7) 2003 (April). Qualified Advanced Course on the safe use of radioactive materials at PGIMER, Chandigarh, India. I learned the techniques involved in the safe use and disposal of radioactive materials in the hospitals and associated clinical and research laboratories in this 6 weeks course.

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Professional Referees Chyung-Ru Wang, Ph.D. Professor

Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University 320 E. Superior Ave, Searle 3-401, Chicago, IL 60611, USA Phone: 312-503-9748

E-mail: chyung-ru-wang@northwestern.edu Prof. G. K. Khuller, Ph.D.

Senior Professor (Former Head of the Department) Department of Biochemistry,

PGIMER, Chandigarh –160 012 [India] Tel Res. – 91-172-5577570, 91-172-2576852 Cell No. – 91-9814204024

E-mail – gkkhuller@yahoo.co.in Dr. J. S. Schorey, Ph.D. Associate Professor,

Department of Biological Sciences, 129 Galvin Life Science Center, Notre Dame, IN 46556, USA Tel – 574-631-3734

E-mail – schorey.1@nd.edu Dr. Suraj B. Sable, D.V.M, Ph.D Immunology Activity Leader,

Mycobacteriology Laboratory Branch, Division of Tuberculosis Elimination, Bildg. 17, Room No 4042, MS-G35,

Centers for Disease Control and Prevention, 1600 Clifton Road, NE,

Atlanta, GA, USA-30333. Phone: 404-639-1478 Fax: 404-639-5484 E-mail – dpt7@CDC.GOV

References

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