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Improved Acetone Butanol Ethanol (ABE) Solution Analysis Using HPLC: Chromatograph Spectrum Deconvolution Using Asymmetric Gaussian Fit

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Figure 1. HPLC chromatograms at (a) 0.5 mL/min, 65˚C; (b) 0.3 mL/min, 80˚C; (c) 0.5 mL/min, 80˚C; (d) 0.8 mL/min, 80˚C; (e) 0.4 mL/min, 85˚C; (f) 0.8 mL/min, 85˚C; (g) 0.9 mL/min, 90˚C; (h) 1 mL/min, 87˚C
Table 1. The standard calibration curves and retention times of ABE solution components in HPLC chromatograms
Table 2. Results of HPLC and the computer program for 10 g/L aqueous binary solutions of glucose, xylose, acetic acid, butyric acid, acetone, ethanol and butanol
Table 3. Repeatability, intermediate precision and reproducibility of the proposed method for all components present in ABE broths in term of coefficient of variation (CV) for retention time and peak area
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