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Hypoxia-inducible factor 1 alpha is regulated by RBM38, a RNA- binding protein and a p53 family target, via mRNA translation

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Figure 1: HIF1α expression is regulated by RBM38 under a hypoxic condition. (A) HCT116 cells were uninduced (-) or induced (+) to express RBM38 with doxycycline for 24 h and then treated with 500 µM CoCl2 for the indicated times
Figure 2: HIF1α expression is increased by knockdown of RBM38 under a hypoxic condition
Figure 3: Knockdown of RBM38 has no effect on the level of HIF1α mRNA. (A-B) H1299 (A) and p53-/- HCT116 (B) cells were transduced with a lentivirus expressing a control luciferase (Luc) shRNA or RBM38 shRNA for 3 d, followed by exposure to hypoxia for 0-6
Figure 4: Knockdown of RBM38 enhances HIF1α expression through mRNA translation. 35S-metabolic labeling assay was performed with H1299 (A) or p53-/- HCT116 (B) cells
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