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Loss of β-catenin in adrenocortical cancer cells causes growth inhibition and reversal of epithelial-to-mesenchymal transition

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Figure

Figure 1: β-catenin expression in adrenocortical tissues and specific silencing of β-catenin mediated by stable expressing β-catenin shRNA
Figure 2: Characterization of in vitroin triplicate, and counted 1, 2, 3, 4 and 5 days later by trypan blue exclusion *P<0.01
Figure 3: Expression of β-catenin, axin2, lef1, p21, p27, p57, p-MAPK and p-Akt in H295R cells treated with β-catenin shRNA
Figure 4: Loss of β-catenin is sufficient for increased apoptosis. Representative FACS histograms indicating the percentage of apoptotic H295R-sh-Cntrl, -sh-βcat-2 and -sh-βcat-3 cells as determined by binding of FITC-conjugated Annexin V.
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