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Endoplasmic reticulum stress cooperates with Toll like receptor ligation in driving activation of rheumatoid arthritis fibroblast like synoviocytes

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Figure

Fig. 1 Effects of ER stress on RA FLS inflammatory gene expression and cellular viability.Glyceraldehyde 3-phosphate dehydrogenase,ODfactor,nM TG for 8, 16, or 24 h or with increasing concentrations of TG for 24 h.after 24-h incubation with 10 nM TG.CHOPqP
Fig. 2 Synergism between ER stress and other stimuli.synoviocytes,TGunstimulated cells for 30 genes with the highest overall level of regulation (mean across all conditions).array
Fig. 3 Synergism between ER stress and multiple TLR ligands. mRNA expression of IL6 (a), IL8 (b), and CXCL10 (c) in RA FLS was measured by qPCR after4-h stimulation with the indicated TLR ligands in the presence or absence of 10 nM TG
Fig. 4 Effects of ER stress on gene transcription and mRNA stability in RA FLS.FLSLPS in the presence or absence of 10 nM TG for 4 h, followed by incubation with 10indicated time points after addition of ActD, and the amount of transcript for each gene rem
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