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Evaluation of a PCR Assay for Quantitation of Rickettsia rickettsii and Closely Related Spotted Fever Group Rickettsiae

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Figure

TABLE 1. Oligonucleotide primer pairs used for development of SQ-PCR assay for R. rickettsii
FIG. 1. Standard calibration curve for SQ-PCR for Rickettsiafragment of with plasmid pCR2.1-TOPO containing the cloned Rr190.547F-Rr190.701R R
TABLE 2. Effect of DNA extraction methods on the quantitation of R. rickettsii by SQ-PCR
TABLE 3. Specificity of SQ-PCR assay for different genotypesof Rickettsia
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