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The effect of 3β, 6β, 16β trihydroxylup 20(29) ene lupane compound isolated from Combretum leprosum Mart on peripheral blood mononuclear cells

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Figure

Fig. 1 Molecular structure of triterpene lupane 3β, 6β, 16β-trihydroxylup-20(29)-ene (TTHL) [7]
Fig. 2 Effect of lupane on PBMC viability after 1 h (a), 15 h (b) and24 h (c) of incubation
Fig. 3 Effect of lupane on the release of TNF-α (a) and IL-10 (b) by PBMCs. PBMCs (2×105) were incubated with lupane at 0.3, 0.7 and 1.5 μg/mLor RPMI (negative control) or LPS (positive control, 1 μg/mL) at 37 °C in a humidified atmosphere of 5 % CO2 for 1
Fig. 4 Effect of lupane on topoisomerases I and II activity. Electrophoresis in 1 % agarose gel to assess the activity of TOPO I (a): nuclear extractsof PBMCs and supercoiled DNA (TopoGen) (control), lupane (6; 1.5 or 0.7 μg/mL plus nuclear extract of PBMC
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