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Denaturation of DNA in Ternary Mixed Solution of Water/Hydrophilic/Hydrophobic Organic Solvent

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Figure

Figure 1. Absorption spectra of λ-DNA in the ternary mixed solution. Conditions: solution, water/acetonitrile/ethyl acetate (15:3:2, volume ratio); standing time, (a) 0, (b) 10, and (c) 48 h; temperature, 23˚C; and λ-DNA concentration, 0.8 nM
Figure 2. Absorbance vs. time for λ-DNA in (a) pure water, (b) water/acetonitrile (15:3, volume ratio), and (c) water/acetonitrile/ethyl acetate (15:3:2, volume ratio)
Figure 3. Absorbance vs. time for plasmid in (a) water, (b) water/acetonitrile (15:3, vo-sorbance on the vertical line is estimated based on the absorbance at 260 nm for the standing time of 0 h
Figure 4. Electrophoretic bands of (1) DNA ladder and 5.5 nM plasmid in Tris-EDTA solution as well as plasmid treated with (a) water, (b) water/acetonitrile (15:3, volume ra-tio), and (c) water/acetonitrile/ethyl acetate (15:3:2, volume ratio)

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