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Mixed Micellar Electrokinetic Chromatographic Analysis of Colistin, Polypeptide Antibiotic, Using Laser Induced Fluorescence Detection

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Figure

Figure 1. Chemical structures of colistin A and B. Fatty acid: 6-methyloctanoic acid for colistin A and 6-methylheptanoic acid for colistin B; Thr: threonine; Leu: leucine; Dab: the peptide linkage
Figure 2. Effect of FITC concentration (a), reaction time (b), and reaction temperature (c) on the FITC-colistin derivati- zation yield
Figure 3. Effect of TX100 on BGB in presence of SDS (2.3 g%) on colistin (5 µg/ml) analysis
Table 2. Inter-day and Intra-day recovery and precision data for the proposed method for CSU assay after FITC derivatization

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