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Mycobacterium tuberculosis infection induces non apoptotic cell death of human dendritic cells

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Figure 1 Dendritic cells mature after they phagocytose M. tuberculosis. A. Human monocytes were separated from buffy coats by plasticadherence and cultured for 6 days in the presence of recombinant human IL-4 (40 ng/ml) and GM-CSF (50 ng/ml) to allow diffe
Figure 2 Liveinfected with live H37Ra at MOI 1, 5 or 10. DNA fragmentation was measured by Cell Death ELISA 72 h after infection
Figure 3 Dendritic cell death afterirradiated H37Rv, STS = staurosporine, CHX = cycloheximide.) *parallel by propidium iodide exclusion(upper panels)
Figure 4 Dying M. tuberculosis-infected DCs secrete cytokines. DCs were infected with live/dead Mtb H37Ra at MOI 1 for 24 h or 48 h, ortreated with LPS (1 μg/ml) for 24 h
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