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Allosteric interactions governing oxygen equilibria in the haemoglobin system of the spiny dogfish, Squalus acanthias

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Figure

Fig. 3. Effects of ATP (•) and GTP (A) on Pm and n» of the stripped haemolysate measured at15°C, pH7-28 in 010M-Na-Hepcs buffer
Fig. 5. Effects of urea on Oi equilibria of the stripped haemolysate measured at ISin the absence (open symbols) and presence (closed symbols) of 0'42 M-urea, in 0-1 M-Na-Hepcsbuffer
Fig. 6. Dependence of PnHepes buffer. Haem concentration, 0-30ratio, and nx of the stripped haemolysate on pH in the absence of added ligands(O) and in the presence of ATP (•), urea (D) and ATP + urea (•) measured at 15 °C in 0' 1 M-Na- ITIM; urea (where present), 0-46M; ATP/Hb (mol/mol) 1-51.
Fig. 8. Isolation of component Hbs by isoelectric focusing in solutions of ampholines (LKB,Sweden) with pH ranges of 5 to 8 (0-42%) and 3-5 to 10 (0-28%)

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