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dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinases

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Academic year: 2020

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Figure

Figure 1 Expression and transactivation of dCas9.nVP64.Notes: (A) Cartoon illustration of the HA-tagged deactivated-form of dCas9.nVP64 was engineered at the N-terminus in the transactivating construct (dCas9.nVP64) while the non-transactivating construct
Figure 2 Loci-specific transactivation of the mouse TIMP promoters using dCas9.nVP64.Abbreviation:Notes: Fold-induction over the no gRNA transfection controls using dCas9.nVP64 (black circle) or dCas9 (orange triangle) and the relative gRNAs targeting loci
Figure 3 Comparison of the transactivation efficiency of TIMP1 and TIMP2 promoters using different transcriptional activating effectors.Notes: (A) Illustration of the different dCas9 constructs used
Figure 4 Endogenous TIMP gene activation in mouse NSC34 cells.Notes: (A) PMA stimulation enhances promoter-reporter activity for TIMP1 and 3
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