CONFERENCE PROGRAMME

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CONFERENCE PROGRAMME

Start

Time MONDAY, 14th December, Conference Room, John Paul II Hotel, 2 Św. Idziego Street 7:30am -

5:30pm Registration

8:30am - 8:45am

Introductory remarks. Andrzej Lange, L. Hirszfeld Institute of Immunology and Experimental Therapy PAS/ Lower Silesian Center for Cellular Transplantation, Wroclaw, Poland

8:45am - 9:30am

Introductory lecture: Novel view on hierarchy of adult bone marrow stem cells and mechanisms regulating stem cell trafficking. Mariusz Z. Ratajczak, University of Louisville, USA/ Pomeranian Medical University, Poland

9:30am - 1:45pm

Session 1. Mechanisms involved in somatic stem cell quiescence and mobilization Chairs: Mariusz Z. Ratajczak, Edward Scott

9:30am - 10:15am

Not all Hematopoietic Stem Cells regenerate equally: How HSC regulate scarification versus regeneration in vertebrate species. Edward W. Scott, University of Florida, Gainesville, USA

10:15am -

11am

Metabolic regulation of blood and bone forming stem cells by ROS and by Nitric Oxide. Tsvee Lapidot, Weizmann Institute of Science, Rehovot, Israel

11am -

11:15am coffee break

11:15am -

12pm

Mesenchymal stem cells of different tissue origin: the crosstalk between the MSCs and their local microenvironment. Diana S. Bugarski, University of Belgrade, Serbia

12pm -

12:45pm

The effects of pituitary-derived hormones on human stem and progenitor cell subsets. Marcin Moniuszko, Medical University of Bialystok, Poland

12:45pm – 6:15pm

Session 2. Organ specific stem cells

Chairs: Marcin Moniuszko, Graca Almeida-Porada

12:45pm - 1:30pm

Plasticity of adult stem cells in endocrine and neural reconstitution. Nadir Askenasy, Schneider Children’s Medical Center of Israel, Petach Tikva, Israel

1:30pm - 2:15pm

Ependymal stem cells based therapy. Miodrag Stojkovic, University of Kragujevac, Faculty of Medical Sciences, Centre for Molecular Medicine and Stem Cell Research, Kragujevac, Serbia

2:15pm –

3:45pm Lunch & Posters

3:45pm – 4:30pm

Identification and modification of bone marrow-derived stromal cells for regenerative medicine. Graca Almeida Porada, Wake Forest University School of Medicine, USA

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4:30pm - 5:15pm

Skeletal Stem Cells: Targets for enhancing bone formation. Moustapha Kassem, University Hospital of Odense, Denmark

5:15pm – 6:00pm

Human Mesenchymal Stromal Cells: Biological Properties and Clinical Application. Erdal Karaoz, Liv Hospital, Istanbul, Turkey

7pm -

11pm

Take Together to Appreciate Participants, Concert & Gala Dinner, Town Hall, Piwnica Świdnicka,14/15 Sukiennice Street

Concert Program:

Wacław z Szamotuł / Modlitwa, gdy dziatki spać idą Vytautas Miškinis / O sacrum convivium

Arvo Pärt / Bohoroditse Djevo

Felix Mendelssohn-Bartholdy / Richte mich Gott Moses Hogan / Hold on

Camille Saint-Saëns / Oratorio de Noel

ARS CANTANDI - WROCLAW UNIVERSITY of ECONOMICS CHOIR

Conductor: Anna Grabowska – Borys; Assistant: Magdalena Truszkowska – Reut Monika Piechaczek (soprano)

Marta Kilian (mezzosoprano) Marta Motkowicz (alto) Przemysław Borys (tenor)

Jerzy Butryn (bass)

Marta Komorowska (piano/organ) Start

Time TUESDAY, 15th December, Conference Room, John Paul II Hotel, 2 Św. Idziego Street 7:30am -

5:30pm Registration

8am –

12am

Session 3. Adult Tissue-Derived Mesenchymal Stem Cells Chairs: Barbara Łukomska, Hiroshi Mizuno

8am -

8:45am

Mesenchymal stem cells: possibilities and limitations in the application. Jasmin Nurkovic, State University of Novi Pazar, Novi Pazar, Serbia

8:45am - 9:30am

Regulation of inflammatory processes by bone marrow derived stromal cells. Eva Mezey, National Institutes of Health, Bethesda, USA

9:30am - 10:15am

Glioblastoma-mesenchymal stem cell communication modulates expression patterns of kinin receptors: possible involvement of bradykinin in information flow. Henning Ulrich, University of São Paulo, Brazil

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10:15am –

11:00am

Direct and/or indirect effect and the role of adipose-derives stem cells for tissue repair and regeneration. Hiroshi Mizuno, Juntendo University School of Medicine, Tokyo, Japan

11:00am - 11:45am

Mechanisms of action and bone forming capacity of human bone marrow mesenchymal stromal cells are donor dependent. Nathalie Chevallier, University of Paris-EST-Créteil, France

11:45am

– 12am coffee break

12:00pm – 1:30pm

Oral presentations

Chairs: Aleksandra Klimczak, Diana S. Bugarski

The effect of oral S1P receptor modulator (FTY20) on stem and progenitor cell mobilization in a murine model of sever colitis: implications for potential clinical application of FTY20. Wojciech Marlicz | Karol Serwin | Ewa Kubiś | Sylwia Rzeszotek | Magdalena Lewandowska | Elżbieta Urasińska | Mariusz Z. Ratajczak

Presenter: Wojciech Marlicz, Pomeranian Medical University, Szczecin, Poland

Presenter: Marta Rasmus, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Poland

The possibility of using the CD271+ and CD271- sub-populations of mesenchymal stem cells in intervertebral discs regeneration. Katarzyna Jezierska-Woźniak | Paweł Wojtacha | Aleksandra Habich | Krzysztof Bladowski | Monika Barczewska | Joanna Wojtkiewicz | Wojciech Maksymowicz Presenter: Katarzyna Jezierska-Woźniak, University of Warmia and Mazury, Olsztyn, Poland

Isolation and phenotypical differences of MSC obtained from human bone marrow and skeletal muscle. Urszula Kozłowska | Tomasz Jurek | Magdalena Czuba | Marta Rorat | Aleksandra Klimczak

Presenter: Urszula Kozłowska, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

Intravenous injected mesenchymal stem cell-derived extracellular vesicles are localized in site of ischemic injury and improve blood flow recovery in vivo. Anna Łabędź-Masłowska | Luisa Vergori | Sylwia Kędracka-Krok | Elżbieta Karnas | Michał Sarna | Ramaroson Andriantsitohaina | Ewa K. Zuba-Surma

Presenter: Anna Łabędź-Masłowska, Jagiellonian University, Krakow, Poland

Persenter: Anna Andrzejewska, Mossakowski Medical Research Centre, PAS, Warsaw, Poland

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Haematopoietic fresh stem cells CD34 positive and joint cartilage treatment. Marek Krochmalski | Witoński Dariusz | Grzelak Piotr

Persenter: Marek Krochmalski, Medical Magnus Clinic Lodz, Poland

1:30pm -

3:00pm Lunch & Posters

3:00 pm –

5:45 pm

Session 4. Plasticity of stem cells and practical aspects of clinical translation Chairs: Ewa Zuba-Surma, Miodrag Stojkovic

3:00am-3:45pm Better Manufacturing Practice for Cell Therapies, Kevin Murray, Biospherix, USA

3:45 pm – 4:15pm

Hematopoietic stem/progenitor cells express several functional sex hormone receptors - novel evidence for a potential developmental link between hematopoiesis, VSELs and primordial germ cells. Magdalena Kucia, University of Louisville, Louisville, KY, USA

4:15pm –

5pm

Molecular and functional properties of murine and human VSELs – continuum. Ewa Zuba-Surma, Jagiellonian University, Krakow, Poland

5pm –

5:45pm

Regeneration of Anti-Leukemic Activity in Patients Relapsing After AlloHSCT: The Possible Effect of Anti-Tyrosine Kinase Inhibitors Andrzej Lange, L. Hirszfeld Institute of Immunology and Experimental Therapy PAS, Lower Silesian Center for Cellular Transplantation, Wroclaw, Poland

Start Time WEDNESDAY, 16th December, WORKSHOPS GMP Facility/Scientific-Medical Incubator of the Lower Silesian Centre for Cellular Transplantation 6 Gen. Augusta Emila Fieldorfa Street/ 18 Muchoborska Street

9am-9:15am

Introduction, organizational aspects of workshops – Andrzej Lange, L. Hirszfeld Institute of Immunology and Experimental Therapy PAS/ Lower Silesian Center for Cellular Transplantation, Wroclaw, Poland

9:15am -

10am

GMP facility – how it looks like. Andrzej Lange, Agata Borowik, Jolanta Bochenska Lower Silesian Center for Cellular Transplantation, Wroclaw, Poland

10:20am -

1:30pm Mesenchymal stem cells propagation. Stefano Baila, TERUMO BCT

We describe good manufacturing practices-compliant procedures for the production of ATMPs using the Quantum Cell Expansion System. The Quantum system is closed and automated medical device that has been successfully implemented in different manufacturing processes, form preclinical to clinical phases. Presented cases will address challenges and opportunities for cell and gene therapies manufacturing in the context of a closed and automated system. Additionally we will present how the system allows large-scale expansion of ATMPs while maintaining the critical quality attributes of interest.

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1:30pm -

1:45pm coffee break

1:45pm -

4pm

Expanding T cells for therapy in a closed bioreactor system. Walter Kohler, Cell Therapy Technologies

T cell therapies are a rapidly growing field of person medicine, but the large numbers of cells required present a logistical and technical challenge. Growing T cell in a XuriTM cell expansion system offers the benefits of being a closed, single-use and automated system in which high cell densities can be reached. Monitoring cell growth requires a sample to be taken from the culture, which briefly compromises the closed environment and risks the sterility of the culture. We show here how dissolved oxygen concentration acts as a surrogate readout for cell growth.

10am -

5pm Multi-omic – transcriptomics & proteomics analysis. Danielle Fletcher, Agilent Technologies.

During the workshop Danielle Fletcher will present unique features of Agilent’s GeneSpring software that allow the combined analysis of transcriptomics and proteomics data. Participants will work on exemplary dataset and will conduct the complete workflow to obtain an integrated view on certain biological process. The aim of the workshop is to demonstrate that with proper bioinformatics tools the systems biology data analysis is at the reach of user’s hand.