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Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study

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Academic year: 2020

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Figure

Fig. 1 SDS-PAGE analysis of recombinant protein lipBJ10 sample from BL21 (DE3) containing different recombinant plasmid from pET-SigPFL00 to pET-SigPFL06 induced with 0.2 mM IPTG for 40 h at 20 °C
Fig. 2 Relative expression level of lipBJ10 in different recombinant between different strains were statistically significant at a strains (from BL21-00 to BL21-06)
Fig. 4f, i clearly demonstrate that most IBs (black arrow) tend to evenly distribute around the interior of the cell
Fig. 5 Comparison of the lipase activity of cytoplasmic protein preparation and whole-cell preparation
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