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Gene Expression of Promyelocytic Leukemia Proteins and IFN-γ Is Reduced in Rotavirus-Infected Children

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Figure

Fig. 1. Agarose gel electrophoresis of PCR-amplified products of rotavirus species-specific primers (NSP3) using: (A) 2% agarose:lane 1, 100 bp DNA size marker; lane 2, no template (negative) control; lanes 3-8 the examined sample
Fig. 2. Melting curve analyses of PML, PML-II, IFN-γ, and β-actin. Fluorescence change was measured with increasing temperature to separate the ds of the DNA to form single-stranded DNA
Fig. 3. Representative amplification curves of PML, PML-II, IFN, and β-actin. Amplification curves were generated by plotting the cycle number at the crossing point with the threshold value versus the log of starting amount of template
Fig. 4. RT and real-time PCR analysis of PML (A), PML-II (B), and IFN-γ (C) mRNA relative expression in positive and negative samples

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