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Molecular and Morphological Data Support the Existence of a Sexual Cycle in Species of the Genus Paracoccidioides

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Figure

FIG 1 MATphylogenies generated by the maximum parsimony method are shown and allow discrimination betweenusing both theidentification inIdiomorphic distribution of 98 isolates revealing that 56% harbored locus amplification, population distribution, and phyl
FIG 2 Quantitative expression of the sex-related genes MAT1-1 (�-box), MAT1-2 (HMG), ste2 and ste3 (pheromone receptors), fus3 (MAPK), and ste12(transcriptional factor) in yeast and mycelial phases of the homothallic strain Pb18 (A) and the heterothallic strains Pb3 (B), Pb01 (C), T1F1 (D), EE (E), T9B1(F), and T15LN1 (G).
FIG 3 Pheromone stimulation after coculture of strains of opposite matinging YEM medium (CM)
FIG 4 Initial stages of sexual development in Paracoccidioides species. The sexual structures were obtained by mating experiments using V8, SEA, or OTAmedium, and the samples were monitored for 6 months
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