Brochure More information from

Full text

(1)

Brochure

More information from http://www.researchandmarkets.com/reports/2172993/

Proteomics Today. Protein Assessment and Biomarkers Using Mass

Spectrometry, 2D Electrophoresis,and Microarray Technology. Wiley Series

on Mass Spectrometry

Description: The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.

- Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome

- Explains the hows and whys of using mass spectrometry in proteomic analysis

- Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis

- Comments on future challenges and how they may be addressed - Includes sections on troubleshooting

Contents: PREFACE TO PART I. ACKNOWLEDGMENT. I: INTRODUCTION TO PART I.

1. INSTRUMENTATION AND DEVELOPMENTS. 1.1 Introduction.

1.2 Ionization Techniques for Macromolecules.

1.3 Examples on Analytical Solutions Based on FAB–MS. 1.4 Electrospray Ionization.

1.5 Matrix-Assisted Laser Desorption Ionization. 1.6 Ion Detection.

1.7 Types of Analyzers. 1.8 Hybrid Analyzers.

1.9 Tandem Mass Spectrometry.

1.10 Current MS Instrumentation in Proteome Analyses. 1.11 Current MS-Based Proteomics.

1.12 Recent Achievements and Future Challenges. 1.13 Concluding Remarks.

References.

(2)

2.1 Introduction.

2.2 Pancreatic Ductal Adenocarcinoma.

2.3 Proteomic Analysis of Human Breast Carcinoma. 2.4 Proteomic Profiling of Chemoresistant Cancer Cells. 2.5 Signal Pathway Profiling of Prostate Cancer.

2.6 Emerging Role of Functional and Activity-Based Proteomics in Disease Understanding. 2.7 Activity-Based Protein Profiling.

2.8 Probing Protein Functions Using Chromophore-Assisted Laser Inactivation. 2.9 Role of Protein–Tyrosine Kinases.

2.10 Concluding Remarks and Future Prospects. References.

3. CURRENT STRATEGIES FOR PROTEIN QUANTIFICATION. 3.1 Introduction.

3.2 Global Internal Standard Technology. 3.3 Differential In-Gel Electrophoresis. 3.4 Quantification of Modified Proteins. 3.5 Comments and Considerations. 3.6 Other Approaches.

3.7 Emerging Role of Microfluidic Devices. 3.8 Concluding Remarks.

References.

II: PROTEOMICS TODAY: SEPARATION SCIENCE AT WORK.

4. CONVENTIONAL ISOELECTRIC FOCUSING IN GEL MATRICES AND CAPILLARIES AND IMMOBILIZED pH GRADIENTS.

4.1 Introduction.

4.2 Conventional Isoelectric Focusing in Amphoteric Buffers. 4.3 Immobilized pH Gradients.

4.4 Capillary Isoelectric Focusing.

4.5 Separation of Peptides and Proteins by CZE in Isoelectric Buffers. 4.6 Conclusions.

References.

(3)

5.1 Introduction.

5.2 SDS–Protein Complexes: a Refinement of the Model. 5.3 Theoretical Background of Mr Measurement by SDS–PAGE. 5.4 Methodology.

5.5 Gel Casting and Buffer Systems. 5.6 Blotting Procedures.

5.7 Conclusions. References.

6. TWO-DIMENSIONAL MAPS. 6.1 Introduction.

6.2 Some Basic Methodology Pertaining to 2D PAGE. 6.3 Prefractionation Tools in Proteome Analysis. 6.4 Multidimensional Chromatography Coupled to MS. 6.5 Protein Chips and Microarrays.

6.6 Nondenaturing Protein Maps.

6.7 Spot Matching in 2D Gels via Commercial Software. 6.8 Conclusions.

References. INDEX.

Ordering: Order Online - http://www.researchandmarkets.com/reports/2172993/

Order by Fax - using the form below

Order by Post - print the order form below and send to Research and Markets,

Guinness Centre, Taylors Lane, Dublin 8, Ireland.

(4)

Page 1 of 2

Fax Order Form

To place an order via fax simply print this form, fill in the information below and fax the completed form to 646-607-1907 (from USA) or +353-1-481-1716 (from Rest of World). If you have any questions please visit

http://www.researchandmarkets.com/contact/

Order Information

Please verify that the product information is correct.

Product Format

Please select the product format and quantity you require:

* Shipping/Handling is only charged once per order.

Contact Information

Please enter all the information below in BLOCK CAPITALS

Product Name: Proteomics Today. Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis,and Microarray Technology. Wiley Series on Mass Spectrometry Web Address: http://www.researchandmarkets.com/reports/2172993/

Office Code: SC

Quantity

Hard Copy (Hard

Back): USD 155 + USD 29 Shipping/Handling

Title: Mr Mrs Dr Miss Ms Prof

First Name: Last Name:

Email Address: * Job Title: Organisation: Address: City:

Postal / Zip Code: Country:

Phone Number: Fax Number:

(5)

Page 2 of 2

Payment Information

Please indicate the payment method you would like to use by selecting the appropriate box.

Please fax this form to:

(646) 607-1907 or (646) 964-6609 - From USA

+353-1-481-1716 or +353-1-653-1571 - From Rest of World

Pay by credit card: You will receive an email with a link to a secure webpage to enter yourcredit card details.

Pay by check: Please post the check, accompanied by this form, to: Research and Markets,

Guinness Center, Taylors Lane, Dublin 8, Ireland.

Pay by wire transfer: Please transfer funds to:

Account number 833 130 83

Sort code 98-53-30

Swift code ULSBIE2D

IBAN number IE78ULSB98533083313083 Bank Address Ulster Bank,

27-35 Main Street, Blackrock, Co. Dublin, Ireland. If you have a Marketing Code please enter it below:

Marketing Code:

Please note that by ordering from Research and Markets you are agreeing to our Terms and Conditions at http://www.researchandmarkets.com/info/terms.asp

Figure

Updating...

Related subjects :