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Akt (Phospho-Ser473) Antibody, Rabbit Polyclonal Antibody

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GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 sales@genwaybio.com

Akt (Phospho-Ser473) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-785-210009

Related Product Names:

- Akt antibody; Akt; PKB; RAC; Akt (Phospho-Ser473)

- EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT; RAC-alpha serine/threonine-protein kinase - Gene Information -

Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Akt Gene Name Synonym: PKB; RAC

Gi #: N/A NCBI Acc #: N/A Swiss Prot Acc #: P31749

Length (aa): N/A Mol. Weight (Da): 55686 Chrom Location: N/A Binding Site: Ser473

WB Dilution: 500~1000 IHC Dilution: 50~100 Source/Host: Rabbit

Purity/Purification: Affinity Purified Clonality: Polyclonal

Crossreactivity: Human, Mouse, Rat

Format: In PBS, 0.02% sodium azide and 50% glycerol.

Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw. Stability: 1 year at -20C

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for AKT ANTIBODY:

IHC, WB: Tested

TESTING: (secondary reagents and protocols)

IH ( anti-Akt )

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WB ( anti-Akt )

Akt antibody image

AKT ANTIBODY TARGET DESCRIPTION:

Synonym Names for Akt antibody: Akt; PKB; RAC; EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT; RAC-alpha serine/threonine-protein kinase

Function: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

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C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.

Subunit: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN.

Subcellular Location: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).

Tissue Specificity: In all human cell types so far analyzed.

Domain: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

Ptm: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).

Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Similarity: Contains 1 AGC-kinase C-terminal domain.

Similarity: Contains 1 PH domain.

Similarity: Contains 1 protein kinase domain.

Akt (Phospho-Ser473) reacts with human, mouse, rat. OMIM: 164730; gene. [NCBI / EBI]

Pathways:

KEGG pathway: Adipocytokine signaling pathway 04920

KEGG pathway: Apoptosis 04210

KEGG pathway: B cell receptor signaling pathway 04662

KEGG pathway: Chronic myeloid leukemia 05220

KEGG pathway: Colorectal cancer 05210

KEGG pathway: Endometrial cancer 05213

KEGG pathway: ErbB signaling pathway 04012

KEGG pathway: Fc epsilon RI signaling pathway 04664

KEGG pathway: Focal adhesion 04510

KEGG pathway: Glioma 05214

KEGG pathway: Insulin signaling pathway 04910

KEGG pathway: Jak-STAT signaling pathway 04630

KEGG pathway: MAPK signaling pathway 04010

KEGG pathway: Melanoma 05218

KEGG pathway: Non-small cell lung cancer 05223

KEGG pathway: Pancreatic cancer 05212

KEGG pathway: Prostate cancer 05215

KEGG pathway: Renal cell carcinoma 05211

KEGG pathway: Small cell lung cancer 05222

KEGG pathway: T cell receptor signaling pathway 04660

KEGG pathway: Tight junction 04530

KEGG pathway: Toll-like receptor signaling pathway 04620

KEGG pathway: VEGF signaling pathway 04370

KEGG pathway: mTOR signaling pathway 04150

Reactome Event:Apoptosis 109581

PRODUCT INTERACTIONS:

Akt (Phospho-Ser473) interacts with B-cell lymphoma/leukemia 10, IgG (GenWay Catalog #:

18-785-210009).

Akt (Phospho-Ser473) interacts with Bcl-10, IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Bcl10, IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Bcl10 [151], IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Bcl10, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with Bcl-10 (C-term), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Bcl-10, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with EMTK / ITK /TSK (C-term), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with B-cell CLL/lymphoma 10 (BCL10) Rabbit anti-Human Polyclonal (aa5-19) Antibody, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with B-cell CLL/lymphoma 10 (BCL10) Rabbit anti-Human Polyclonal (N-Terminus) Antibody, IgG (GenWay Catalog #: 18-785-210009).

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18-785-210009).

Akt (Phospho-Ser473) interacts with MOUSE ANTI HUMAN Bcl-10, IgG (GenWay Catalog #:

18-785-210009).

Akt (Phospho-Ser473) interacts with MOUSE ANTI HUMAN Bcl-10, IgG (GenWay Catalog #:

18-785-210009).

Akt (Phospho-Ser473) interacts with MOUSE ANTI HUMAN Bcl-10:RPE, IgG (GenWay Catalog #:

18-785-210009).

Akt (Phospho-Ser473) interacts with CDC37, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with Glycogen synthase kinase-3 beta, Protein (GenWay Catalog #:

18-785-210009).

Akt (Phospho-Ser473) interacts with GSK3-beta, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with GSK3-beta (Phospho-Ser9), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with GSK3-beta (Phospho-Tyr216), IgG (GenWay Catalog #:

18-785-210009).

Akt (Phospho-Ser473) interacts with GSK3-beta (Ab-9), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with GSK3-beta (Ab-216), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with GSK3-beta (Phospho-Ser9) Antibody Blocking Peptide, Peptide

(GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with Rabbit anti GSK3b pS9, IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Phospho-Ser9 GSK3-beta, IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Anti-GSK3 beta pS9, IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with GSK-3beta (C-term), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with GSK3 beta Phospho (pS9), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Glycogen Synthase Kinase 3, Beta (GSK3B) Rabbit anti-Human Polyclonal (pSer9) Antibody, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with ASK1, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with ASK1 (Phospho-Ser83), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with ASK1 (Phospho-Ser966), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with ASK1 (Ab-83), IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with ASK1 (Ab-966), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with ASK1 (Phospho-Ser83) Antibody Blocking Peptide, Peptide

(GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with ASK1 (Phospho-Ser966) Antibody Blocking Peptide, Peptide

(GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with Rabbit anti ASK-1 pS83, IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with Anti-ASK-1 phospho specific pS83, IgG (GenWay Catalog #:

18-785-210009).

Akt (Phospho-Ser473) interacts with MEKK5 / ASK1 (N-term), IgG (GenWay Catalog #: 18-785-210009). Akt (Phospho-Ser473) interacts with MAP Kinase Kinase Kinase 5 (MEKK5/ASK1) (MAP3K5) Rabbit anti-Human Polyclonal (C-Terminus) Antibody, IgG (GenWay Catalog #: 18-785-210009).

Akt (Phospho-Ser473) interacts with RABBIT ANTI HUMAN ASK1 (C-TERMINAL), IgG (GenWay Catalog #:

18-785-210009).

BACKGROUND REFERENCES for AKT ANTIBODY:

Background references for antibody target are not specific to GenWay products [1] Jones P.F., Jakubowicz T., Pitossi F.J., Maurer F., Hemmings B.A.

Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. [2] Matsubara A., Wasson J.C., Donelan S.S., Welling C.M., Glaser B., Permutt M.A.

Isolation and characterization of the human AKT1 gene, identification of 13 single nucleotide polymorphisms

(SNPs), and their lack of association with Type II diabetes.

[3] Coffer P.J., Woodgett J.R.

Molecular cloning and characterisation of a novel putative protein-serine kinase related to the

cAMP-dependent and protein kinase C families.

[4] Coffer P.J., Woodgett J.R.

Eur. J. Biochem. 205:1217-1218(1992).

[5] Delcommenne M., Tan C., Gray V., Rue L., Woodgett J.R., Dedhar S.

Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase.

[6] Alessi D.R., Andjelkovic M., Caudwell F.B., Cron P., Morrice N., Cohen P., Hemmings B.A. Mechanism of activation of protein kinase B by insulin and IGF-1.

[7] Walker K.S., Deak M., Paterson A., Hudson K., Cohen P., Alessi D.R.

Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha.

[8] Kane S., Sano H., Liu S.C.H., Asara J.M., Lane W.S., Garner C.C., Lienhard G.E.

A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain.

[9] Conus N.M., Hannan K.M., Cristiano B.E., Hemmings B.A., Pearson R.B.

Direct identification of tyrosine 474 as a regulatory phosphorylation site for the Akt protein kinase. [10] Ahn J.-Y., Rong R., Kroll T.G., Van Meir E.G., Snyder S.H., Ye K.

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PIKE (phosphatidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: Akt antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Akt antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Akt antibody, all related information, articles, resources about Akt antibody are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

GenWay Biotech, Inc. 6777 Nancy Ridge Drive, San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 Email: sales@genwaybio.com www.genwaybio.com ©1998 - 2011 GenWay Biotech, Inc. - All Rights Reserved

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