A bioinformatic and computational study of myosin phosphatase subunit
diversity
Rachael P. Dippold and Steven A. Fisher
Department of Medicine, Cardiology, University of Maryland Baltimore, Baltimore, Maryland Submitted 10 April 2014; accepted in final form 25 May 2014
Dippold RP, Fisher SA.A bioinformatic and computational study of myosin phosphatase subunit diversity.Am J Physiol Regul Integr Comp Physiol307: R256 –R270, 2014. First published June 4, 2014; doi:10.1152/ajpregu.00145.2014.—Variability in myosin phosphatase (MP) subunits may provide specificity in signaling pathways that regulate muscle tone. We utilized public databases and computational algorithms to investigate the phylogenetic diversity of MP regulatory (PPP1R12A-C) and inhibitory (PPP1R14A-D) subunits. The compar-ison of exonic coding sequences and expression data confirmed or refuted the existence of isoforms and their tissue-specific expression in different model organisms. The comparison of intronic and exonic sequences identified potential expressional regulatory elements. As examples, smooth muscle MP regulatory subunit (PPP1R12A) is highly conserved through evolution. Its alternative exon E24 is pres-ent in fish through mammals with two invariant features:1) a reading
frame shift generating a premature termination codon and 2) a
hexanucleotide sequence adjacent to the 3=splice site hypothesized to be a novel suppressor of exon splicing. A characteristic of the striated muscle MP regulatory subunit (PPP1R12B) locus is numerous and phylogenetically variable transcriptional start sites. In fish this locus only codes for the small (M21) subunit, suggesting the primordial function of this gene. Inhibitory subunits show little intragenic vari-ability; their diversity is thought to have arisen by expansion and tissue-specific expression of different gene family members. We demonstrate differences in the regulatory landscape between smooth muscle enriched (PPP1R14A) and more ubiquitously expressed (PPP1R14B) family members and identify deeply conserved intronic sequence and predicted transcriptional cis-regulatory elements. This bioinformatic and computational study has uncovered a number of attributes of MP subunits that supports selection of ideal model organisms and testing of hypotheses regarding their physiological significance and regulated expression.
myosin phosphatase; Mypt1; Mypt2; CPI-17
THE SEQUENCING of the human genome (44, 104) opened vast
new insights into the variability and diversity encoded within the genome. Before this, predictions for the number of human protein-coding genes ranged between ⬃35,000 (24) and up-wards of 150,000 (14). The count of protein-coding genes in the most recent build of the human genome (GRCh37.p13, 2009) is 20,805, a number that is on par with that of the simple organismCaenorhabditis elegans, 20,532 (WBcel235, 2012). This has led to the premise that much of the complexity of the human transcriptome is derived from the creation of multiple products from a single gene, such that humans have 69,000 different proteins, whereas in comparison the worm has 25,000, according to current database curations (Ensembl, UniProt). Multiple transcripts are generated from a single gene by alternative usage of exons through alternative splicing or
alternative transcriptional start sites, each of which are nearly universal features of multiexon genes in mammals and higher vertebrates (67, 79, 108). An example is the human tropomy-osin ␣(TPM1) gene in which the combination of alternative splicing and multiple transcriptional start sites results in 18 distinct protein coding transcripts (reviewed in Ref. 32).
In humans, the DNA of protein-coding genes accounts for less than 3% of the total genome (20); in the past the remaining 97% of the genome was considered “junk DNA.” However, recent evidence suggests that⬎80% of the genome is biochem-ically active: either transcribed (protein coding, noncoding, and pseudogenes), chromatin-associated, or regulatory (20). These active regulatory regions may be intra- or intergenic and contain cis elements that regulate gene transcription or removal of introns (splicing of exons) to convert pre-mRNA into mature protein coding mRNA. Phylogenetic conservation of noncod-ing DNA sequences has been successfully used to identify transcriptional regulatory elements such as enhancers (re-viewed in Ref. 37), though there is not a simple relationship in either direction between sequence conservation and regulatory elements (reviewed in Refs. 72 and 113). Similarly, it has been found that intronic regions flanking alternative exons, and the cis regulatory elements within, are under selection pressure and often exhibit broad conservation (67).
Tissue specificity and tissue gene expression signatures are also frequently conserved, particularly among mammals and higher vertebrates, such that the transcriptome of a given tissue is more similar across species than it is to other cell types within the same species (3, 6, 67). The evolutionary divergence of multiple closely related family members from a single ancestral gene creates diversity in higher organisms through specific expression, either tissue specific or during develop-ment, and also through specificity of interactions and sub-strates. The differing strategies of cell conservation and diver-sification are illustrated in the protein kinases and phosphatases that regulate approximately one-third of all eukaryotic pro-teins. Approximately 400 serine-threonine kinases are present in the mammalian genome with a steady increase in the number throughout evolution of eukaryotes. In contrast, only ⬃25 corresponding phosphatases are present with little evolutionary change in this number (87). This reflects differing strategies of diversification, as diversity in the activity of Type 1 phospha-tases is generated by a large number and variability within associated regulatory subunits and signaling pathways control-ling their activity (9).
In smooth muscle the myosin light chain kinase (MLCK) and phosphatase (MP) are primary determinants of the state of contraction of the muscle and thereby contribute to the regu-lation of blood flow and pressure and other vital organ func-tions (reviewed in Refs. 34, 38, and 40). MLCK and MP enzymes are also present in striated muscle where their func-Address for reprint requests and other correspondence: S. A. Fisher, S-012
HSFII, 20 Penn St., Baltimore, MD 21201 (e-mail: Sfisher1@medicine. umaryland.edu).
tion is less understood but are thought to play a more modu-latory role in contractile function (reviewed in Refs. 47 and 94), and in nonmuscle cells where they regulate motility and cytokinesis (reviewed in Ref. 65). The MP holoenzyme is composed of three subunits: the protein phosphatase 1 (PP1) catalytic subunit, the MP targeting/regulatory subunit (MYPT), and the small subunit (M21) (reviewed in Ref. 45). A fourth subunit, CPI-17, is an inhibitory subunit that when phosphor-ylated inhibits MP activity (reviewed in Ref. 21).
Using traditional methods, we and others have made some progress in determining how variability in the MP regulatory subunits in humans and animal models may provide cell-specific functions. For example, smooth muscle, like striated muscle, may be functionally dichotomized into fast (phasic) versus slow (tonic) contractile phenotypes. Each uses MCLK and MP for activation and dactivation of force, yet the force outputs and how they are regulated are very different (reviewed in Refs. 27 and 100). Within the Mypt1 regulatory subunit an alternative exon (exon 24) that is included in phasic smooth muscle and skipped in tonic smooth muscle is thought to determine regulation of MP activity by cGK1␣ [nitric oxide (NO) signaling pathway] (51, 83, 120; reviewed in Ref. 15). Similarly, variable expression of an inhibitory subunit of MP, CPI-17 (PPP1R14A), is proposed to determine tissue-specific regulation of MP activity by␣-adrenergic signaling (53, 117; reviewed in Ref. 21).
We hypothesized that through interrogation of publicly available databases we could1) uncover much more about the phylogenetic conservation, variability, and tissue-specific ex-pression of MP subunit isoforms; and 2) discover potential transcriptional and splicing cis-regulatory elements that may control the variability in the expression of MP subunits in muscle tissues, regarding which little is currently understood (reviewed in Refs. 32, 46, 78, and 107). This informatics approach provides new insights into the relationship between MP diversity and muscle diversity and predictions regarding how MP diversity may be generated, providing a foundation for further experimentation and selection of appropriate model organisms.
METHODS
Protein Domain
We used several protein databases to identify conserved protein domains in fly and worm: InterPro (43), PANTHER (102), Pfam (89), and SUPFAM (80).
Conservation and Alignment
Conservation of the Mypt1 alternative exon was determined with the PhastCons conservation track in the UCSC Genome Table Browser (genome.ucsc.edu) (49, 50). All genomic and transcript data were gathered from Ensembl (Release 72, June 2013). Nucleic acid sequence alignment was performed using Clustal Omega (http:// www.ebi.ac.uk/Tools/msa/clustalo/) (98). JalView (112) was used to calculate pairwise sequence identity and to perform alignment editing.
Splice Site and cis-Regulatory Element Predictions
Mypt1⫹2 E24 splice sites were predicted using Human Splicing Finder v2.4.1 (www.umd.be/HSF/) (13) and the Alternative Splice Site Predictor (ASSP; wangcomputing.com/assp) (109), using human and chicken Mypt1 E24 as prediction positive controls. Human Splicing Finder identification of splice sites utilizes a positional
weight matrix (PWM) of consensus splice signals (13) to identify potential splice sites that conform to consensus splice signals above threshold. The ASSP prediction algorithm was developed using se-quences for known constitutive, alternative, and cryptic splice sites to take into account nonconsensus splice sites (109).
Conserved motifs were identified using Gapped Local Alignment of Motifs (GLAM2; meme.nbcr.net) (28). Predicted cis-splicing reg-ulatory elements (SREs) were then identified using Human Splicing Finder v2.4.1 (www.umd.be/HSF/) (13). Human Splicing Finder re-ports predicted SREs from several experimental and computational-based prediction sets: ESE Finder (7, 99), RESCUE ESE (25), and other published predictions (33, 110, 119, 121). Of the experimentally derived SRE prediction sets, exonic splicing silencer (ESS) sequences were obtained by testing random decanucleotide sequences in splicing reporters (110). SREs from ESE Finder and Human Splicing Finder were predicted using consensus motif PWMs derived from SELEX (systematic evolution of ligands by exponential enrichment) experiments (7, 13). The other SRE prediction sets were computa-tionally derived through different parameters: predicted exonic splic-ing enhancers (ESEs) based on hexamer enrichment in weak exons [RESCUE ESE, (25)]; predicted exonic splicing regulatory sequences (ESRs) based on enrichment of conserved (human-mouse) hexam-ers in human-mouse orthologous exons (33); predicted exonic SREs based on enrichment of octamers in constitutive, noncoding exons (121); predicted SREs as asymmetrically enriched in introns (intronic identity elements: IIEs) or exons (exonic identity ele-ments: EIEs) (119).
Expression Data
Affymetrix exon array data (88) and RNA-Seq tissue expression data (85) were obtained from publicly available sources (genome.uc-sc.edu; http://www.ebi.ac.uk/gxa).
Promoter and Enhancer Analysis
Transcriptional start site predictions for human genes were assem-bled from the SwitchGear Genomics Transcriptional Start Site track in the UCSC Genome Table Browser (genome.ucsc.edu) (49, 50). SwitchGear TSS predictions are based on human GenBank cDNAs. UCSC Genome Browser (human, hg19) ENCODE tracks for H3K4Me1, H3K4Me3, and H3K27Ac from 7 human cell lines (19), ENCODE DNase hypersensitivity clusters from 125 human cell types (20, 103), and ENCODE transcription factor ChIP were used to determine regions of transcriptional activity.
Regions conserved between human and mouse were aligned using the ECR Browser (www.dcode.org) (77). Conserved and aligned transcription factor binding sites (TFBS) were identified using the NCBI dcode package including MultiTF and rVista 2.0 (www.dcode.org) (61, 76). The rVista program utilizes the com-prehensive database of TFBS motifs TRANSFAC Pro V10.2 (114) and scores sequence similarity of TFBS to TRANSFAC PWM. We used two cutoffs for TFBS identification:1) the rVista “optimized for function” method that independently optimizes each TFBS to limit the density of an individual TFBS to ⱕ3 sites per 10 kb of random sequence (76) and2) 0.85 fixed cutoff score for sequence similarity to TRANSFAC PWMs.
RESULTS
Phylogenetic Conservation of MP Subunit Families
Catalytic subunit. The PP1 family (PPP1CA, PPP1CB, PPP1CC) is highly conserved with extensive sequence identity between paralogs (⬎85%) and among orthologs in other spe-cies (Fig. 1A; reviewed in Refs. 9 and 10) (70). Orthologs of this family can be found in the fruit fly, worm, yeast, and plants (58), indicating ancient origins for the PP1 catalytic subunit.
The central majority of the PP1 proteins, including the catalytic and binding domains, have nearly identical amino acid se-quences. The modest amount of sequence diversity among the paralogs is found in the distal COOH- and NH2-terminal ends
of the proteins.
Pairwise analysis of the amino acid and nucleotide se-quences indicate that the PPP1CA and PPP1CC paralogs are more similar to each other than to PPP1CB, the gene that codes for the catalytic subunit of MP (also known as PP1or PP1␦). This is true for the fly as well, suggesting that PPP1CB
Ray-finned fishes (including zebrafish)
Ray-finned fishes (including zebrafish) PPP1R14C, Coelacanth
PPP1R14B, Coelacanth
Reptiles and birds (including chicken and lizard) ppp1r14b, Xenopus
ppp1r14c, Xenopus
PPP1R14B, Chinese softshell turtle Amniotes (including chicken and lizard)
Mammals
(including primates and rodents) Placental mammals
(including primates and rodents)
Marsupials Marsupials Placental mammals (including rodents) Placental mammals Apes PPP1R14D, Sloth PPP1R14D, Alpaca Ppp1r14d, Kangaroo rat Ray-finned fish PPP1R14D, Xenopus
PPP1R14A, Anole lizard
Placental mammals (including primates and rodents)
Marsupials Bony fish
ppp1r14a, Xenopus
Bony vertebrates (including zebrafish) Lamprey
C
PPP1R14 C B A D 0.25Reptiles and birds (including lizard)
PPP1R12C, Wallaby
Placental mammals (including primates and rodents)
PPP1R12C, Coelacanth ppp1r12c, Xenopus
PPP1R12C, Platypus
Ciona sea squirts Ray-finned fish (including zebrafish)
ppp1r12a, Spotted gar PPP1R12A, Coelacanth
ppp1r12a, Xenopus Reptiles and birds (including chicken and lizard)
Mammals
(including primates and rodents)
Bony vertebrates (including zebrafish) Bony vertebrates
(including zebrafish)
ppp1r12a, Lamprey
Mammals
PPP1R12B, Coelacanth Reptiles and birds (including chicken and lizard)
PPP1R12B, Platypus
Placental mammals (including primates and rodents)
Marsupials Mbs, Fruitfly Ray-finned fishes (including zebrafish) PPP1R12B, Lamprey
B
PPP1R12 A C B 0.25 Ray-finned fish (including zebrafish) ppp1ca, Xenopus gsp-2, Caenorhabditis elegans Ciona sea squirtsRay-finned fish (including zebrafish) Reptiles and birds (including chicken and lizard)
Placental mammals (including mouse)
Mammals
(including primates and rat)
PPP1CC, Zebra Finch
PPP1CC, Platypus Birds and turtles
ppp1cc, Xenopus PPP1CC, Duck ENSLACG00000008556, Coelacanth PPP1CC, Coelacanth PPP1CA, Platypus Birds Placental mammals Mammals
(including primates and rodents)
PPP1CA, Anole lizard Mammals
Fruitfly PPP1CA, Coelacanth
PPP1CA, Chinese softshell turtle
ppp1cc, Lamprey Ray-finned fish PPP1CB, Tarsier Birds Ray-finned fish (including zebrafish) Mammals Bony vertebrates Placental mammals (including rat) Amniotes
(including mouse and lizard) PPP1CB, Chicken Mammals (including humans) PPP1CB, Coelacanth Bony fish ppp1cbl, Zebrafish
Birds and turtles Placental mammals Lamprey
Vertebrates
Arthropods and nematodes ENSCING00000005405, C.intestinalis
Vertebrates
Bilateral animals (including fly and worm)
GLC7, Saccharomyces cerevisiae
A
PPP1C C A B 0.25originated before vertebrates and invertebrates diverged (58). PP1␥ (PPP1CC) appears to be a newer isoform, having di-verged from PPP1CA, and is less conserved among orthologs than are the PP1␣(PPP1CA) and PP1(PPP1CB). All of the paralogs are ubiquitously expressed.
Thus diversity in the serine-threonine type 1 phosphatases depends on a multitude of PP1 regulatory subunits (reviewed in Ref. 10). The regulatory subunits of the MP catalytic subunit, Mypt1 and CPI-17, have greater evolutionary divergence within their families and greater variability among their or-thologs than the catalytic subunit (Fig. 1).
Regulatory subunit. The Mypt (PPP1R12) regulatory sub-unit family is part of a very large super family of ankyrin repeat domain proteins composed of two subtrees. Along with the PPP1R12 family, the first subtree contains the PPP1R16 (A-B) family, also known as Mypt3 (PPP1R16A) and TGF- -inhib-ited membrane-associated protein (TIMAP; PPP1R16B). The second subtree within this super family contains PPP1R13B, which codes for apoptosis-stimulating protein of p53 1 (ASPP1), PPP1R13L, which codes for inhibitor of apoptosis stimulating protein of p53 (IASPP) protein, and the tumor protein p53 binding protein (TP53BP2). These subfamily members are sometimes classified as Mypts; given their evo-lutionary distance and lack of cardinal features of Mypt family members (described below) we consider them to be distinct subfamilies.
The myosin phosphatase regulatory subunit family of proteins is composed of Mypt1, Mypt2, and MBS85 (PPP1R12A-C, respectively). The PPP1R12 family is well conserved and there is an orthologous Mypt gene in both the fly (Mbs,Drosophila melanogaster) and worm (Mel-11,C. elegans) (68, 116). Each of the PPP1R12 family members contains an RVxF motif (PP1 binding site) and 7 or 8 ankyrin repeat domains toward the NH2-terminus, 1 or 2 conserved Thr phosphorylation sites, and
a leucine zipper motif at the COOH-terminus (reviewed in Ref. 34). Whereas Mypt family members (paralogs) overall have 39 – 61% amino acid identity, the described conserved domains have much higher sequence identities (50 –90%) (reviewed in Ref. 45). These characteristic domains of the Mypt family hold true for the fly and worm orthologs as well (68, 116). The COOH-terminus leucine zipper motif is highly conserved among species and is similar in sequence between family members (88%) (34, 45). BLAST searches of the genome with any of the three PPP1R12 family LZ motifs match only to the other members of the family, suggesting that these specific LZ motif sequences are specific to the PPP1R12 Mypt family.
Inhibitory subunit. Sequence similarity between inhibitory subunit family member paralogs and orthologs is much less
than for the catalytic subunit family (Fig. 1C). However, within the phosphatase inhibitory domain there is ⬎41% sequence similarity (reviewed in Ref. 21). Importantly, the Thr38 phos-phorylation site of PPP1R14A (CPI-17), which regulates its inhibitory activity, and the surrounding residues, which are necessary for phosphorylation of Thr38, are conserved in the other three family members (PPP1R14B-D). Orthologs of PPP1R14 are found in lower species, CG17124 in the fly and F55C10.5 in the worm (64, 95) (flybase.org; wormbase.org), and were not previously identified (21). The Thr38 phosphor-ylation site is conserved in the fly and worm orthologs, while the flanking sequences contain hydrophobic and basic residues but vary from the vertebrate consensus sequence (basic-hydro-phobic-Thr-hydrophobic-basic).
Interestingly, the chicken genome lacks the PPP1R14A gene, and it has been suggested that chicken smooth muscle MP is inhibited by the CPI-17 paralog PHI (PPP1R14B) (17, 18, 54). However, PPP1R14B is also not found in the com-pleted sequence of the chicken genome (Genome assembly Galgal4, Ensembl Genebuild updated Dec. 2013). Another member of the PPP1R14 family, e.g., the more closely related kinase enhanced phosphatase inhibitor (KEPI) (PPP1R14C), may function as the MP inhibitory subunit in chicken smooth muscle.
Alternative Splicing of Regulatory Subunits and MP Diversity
Mypt1. The mammalian PPP1R12A (Mypt1) gene consists of 26 exons, 3 of which are alternatively spliced (E13, E14, and E24). Alternative splicing in the central region of Mypt1 is conserved, though the specific alternative exons vary (are not orthologous): E12 in the chicken (16, 96), E9 and E11 in the worm (115) (wormbase.org), and the functional significance of these variants is unknown. Alternative splicing of Mypt1 E24, a 31 nt exon, determines the coding for, or lack of, a COOH-terminal LZ motif in the Mypt1 protein (Fig. 2D). The LZ motif of Mypt1 is thought to be required for LZ-mediated heterodimerization with cGK1␣- and cGMP-dependent activa-tion of MP (42, 51, 101) (57). The expression of Mypt1 E24 and central splice variant isoforms has been studied extensively during development (51, 83, 97) and in disease models (16, 29, 35, 62, 84, 97, 120) (reviewed in Refs. 15 and 27).
Mypt1 E24 is located in a region of high conservation spanning approximately 120 bp upstream through 200 bp downstream of E24 (Fig. 2A) (97). E24 is partially conserved in fish genomes (tetraodon, fugu, stickleback, medaka, and zebrafish), though, notably, a homologous sequence is absent
Fig. 1. Phylogenetic trees of myosin phosphatase subunit families.A: phylogenetic tree of the protein phosphatase 1 (PP1) catalytic subunit family demonstrates high conservation amongst orthologs and paralogs. The node containing the human myosin phosphatase (MP) catalytic subunit, PPP1CB (PP1), is shown in red, whereas paralogs PPP1CA (PP1␣) and PPP1CC (PP1␥) are in blue.B: phylogenetic tree of the MP targeting subunit family is given with the node containing the human smooth muscle MP subunit, protein phosphatase 1 regulatory subunit 12A (PPP1R12A) (MP targeting subunit 1; Mypt1) shown in red and paralogs PPP1R12B (Mypt2) and PPP1R12C (myosin binding subunit 85; MBS85) shown in blue.C: phylogenetic tree of the MP inhibitory subunit family (PP1 regulatory subunit 14; PPP1R14) demonstrates greater variability between paralogs and orthologs compared with the catalytic subunit inA. The node containing the human MP inhibitory subunit PPP1R14A (c-kinase potentiated protein phosphatase 1 inhibitor 17; CPI-17) is shown in red, whereas the paralogs PPP1R14B (phosphatase holoenzyme inhibitor; PHI), PPP1R14C (kinase enhanced phosphatase inhibitor; KEPI), and PPP1R14D (gastric brain phosphatase inhibitor; GBPI) are shown in blue. In the trees the red boxes represents gene duplication and the dark blue boxes speciation events. Light blue boxes are ambiguous nodes. Branch lengths along the horizontal axis correspond to the expected number of changes per nucleotide site in the DNA, as indicated in the corresponding scale bars, which is proportional to evolutionary divergence. The length (horizontal) of solid branches and nodes (triangles) is 1⫻distance, whereas dashed branches and striped nodes are 10⫻distance. The width (vertical) of nodes is proportional to the number of orthologs included in the node. Phylogenetic trees were developed by Ensembl, Release 75 (February 2014) (105).
in the frog, lamprey, and C. elegans orthologs. Sequence alignment of the alternative exon and 50 bp flanking regions demonstrates that this region is highly conserved in mammals, the chicken, and lizard; in fish there is 67–73% sequence identify of E24 to mammals, whereas the flanking sequence is less well conserved than in the higher vertebrates (Fig. 2B).
The 3= and 5= splice sites are computationally predicted in the fish flanking the sequence that is homologous to mamma-lian Mypt1 E24 alternative exon, resulting in a 34 (most fish) or 37 nt (zebrafish) predicted exon (Fig. 2B). Using RT-PCR, we confirmed that this is indeed a tissue-specific alternative exon in the zebrafish smooth muscle (data not shown). As in higher vertebrates the fish alternative exon also causes a 1-nt
shift in the reading frame resulting in a Mypt1 subunit that lacks the COOH-terminal LZ motif (Fig. 2D, right). The LZ motif is highly conserved in the annotated PPP1R12A protein product in tetraodon, fugu, and zebrafish (Fig. 2D,left). The shift in the reading frame also results in a premature termina-tion codon (PTC) in the fish Mypt1, a feature that is also conserved throughout phylogeny (Fig. 2D,right), suggesting functional importance. However, the function and significance of the Mypt1 COOH-terminus alternative (LZ⫺) amino acid sequence and PTC, respectively, are not known at this time.
The intronic sequence immediately flanking the alternative exon is well conserved (Fig. 2B). The nonconsensus 5=splice site sequence (guagua) and the lack of an upstream polypy-Mypt1 E24 Mammal Cons
A
B
0 1 2 bits 1T
G
2C
3T
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10T
11 0 1 2 bits 1G
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9 0 1 2 bits 1 CA
2T
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4C5G
6T
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19 0 1 2 bits 1A
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9 human mouse chicken lizard tetraodon fugu medaka stickleback zebrafishC
SRSF2SRSF6 3’ splice signal 5’ splice signal
SRSF6
D
fugu * * * * * * * human mouse chicken lizard tetraodon zebrafish E24-out E24-in human mouse chicken lizard tetraodon fugu zebrafish LZ+ LZ-chr12, -1: hg19 80,172,900 80,173,000 80,173,100 80,173,200 80,173,300 Vertebrate Cons predictedFig. 2. Phylogenetic conservation of isoforms of Mypt1 generated by alternative splicing of Exon 24.A: conservation of the myosin targeting subunit 1 (Mypt1) alternative exon E24 and the flanking intronic region is shown by the phastCons track on the UCSC Genome Browser in the human hg19/GRCh37 genome release. The phastCons track is a multiple alignment of 46 vertebrate species (“Vertebrate Cons”) and a subset of 33 placental mammal (“Mammal Cons”) that estimates the probability of individual nucleotides belonging to a conserved element, by considering both the individual alignment column and its flanking columns. The higher the green bars the more likely the region belongs to a conserved element.B: alignment and coloring based on percent identity of Mypt1 E24 in human, mouse, chicken, and lizard, and the predicted alternative exon in fish demonstrates regions of high conservation immediately flanking the exon. Red lines attached to triangles highlight known and predicted (in fish) splice sites. Conserved sequences were identified and analyzed for splicing cis-regulatory elements as described inMETHODS.C: exon-intron structure of the 3=end of the Mypt1 gene is shown. Alternative splicing of the 31 nt E24 changes the reading frame. Amino acid sequence alignment of the Mypt1 COOH-terminus for the E24-out/leucine zipper (LZ)⫹and the E24-in/LZ⫺isoforms, demonstrates phylogenetic conservation, with LZ⫹more conserved than LZ⫺. The leucines of the LZ motif for the E24-out isoform (left) are highlighted in gray. The amino acids that are coded by the alternative exon E24 are highlighted in blue.
rimidine tract are both characteristic of a weakly spliced (alternative) exon (reviewed in Ref. 2). Two upstream and one downstream blocks of intronic sequence are highly conserved from fish through higher vertebrates suggesting they could function in the regulation of exon splicing (Fig. 1B). Analysis of these sequences using splicing regulatory elements (SRE) prediction algorithms for known cis-splicing regulatory ele-ments (seeMETHODS) reveals conserved sites for splicing factors
SRSF2 (SRp30b) and SRSF6 (SRp55) in the intronic regions immediately flanking E24 (Fig. 2C). We also identified com-putationally predicted SREs of unknown function (25, 119) within the highly conserved sequence immediately adjacent to the 3= splice site: ctgaaa (human-lizard)/ctgaag (fish) and tgaaag (human-lizard)/tgaagG (fish) (Fig. 2C). The proximity of the identified elements to the 3=splice site suggests that they may function as splicing repressors by blocking recruitment of U2 splicing factor to this site (seeDISCUSSION). Of note the E24
sequence itself is highly conserved in higher vertebrates but less well conserved in fish. A number of cis-regulators of splicing located within higher vertebrates’ E24 (97) are not present within the fish E24 sequence. How this may affect the regulation of E24 splicing is considered further in DISCUSSION.
A number of other predicted conserved cis-regulatory splicing elements are identified both within the alternative exon and flanking introns (Supplemental Table S1).
Mypt2.Alternative splicing of exon 24 (numbering based on human gene) of Mypt2 (PPP1R12B) gene product has similar-ities and differences with that of Mypt1 E24 but has been much less studied. The Mypt2 E24 skipped isoform codes for a highly conserved COOH-terminal LZ motif (Fig. 3C) that is nearly identical in amino acid sequence to the COOH-terminal LZ sequence of family members Mypt1 and p85. In contrast to Mypt1 E24, Mypt2 E24 inclusion codes for an alternative COOH-terminal LZ sequence and contains the PTC (Fig. 3A). Thus MBS85 (PPP1R12C) is the only Mypt family member with an invariant COOH-terminal (LZ) sequence. Like Mypt1 E24, splicing of Mypt2 E24 is highly restricted (described in detail under Complexity of the Mypt2 Locus).
Like Mypt1 E24, Mypt2 E24 sequence is highly conserved in the genomes of mammals (94% sequence identity to mouse), chicken (76%), and lizard (74%) and also absent in frog. There is 58 – 63% conservation of the coding portion of the exon of the human Mypt2 E24 in fish (medaka, stickleback, tetraodon) (Fig. 3B). Unlike Mypt1, there is a polypyrimidine-rich tract upstream of Mypt2 E24 and high 3= splice site consensus conformity (85–91%) in all of the species examined, giving a robust 3= splice site prediction conforming to the known mammalian splice site (Fig. 3B, red line). The stop codon is also in alignment, though it is a TGA in fish as opposed to a TAG in mammals, chicken, and lizard (Fig. 3B, red asterisk). There is minimal homology in the exonic sequence immedi-ately downstream of the PTC, and the downstream intronic flanking region is not conserved. The annotated mammalian 5= splice site (Fig. 3B, red line) was not predicted by either the Human Splicing Finder (13) or the Alternative Splice Site Predictor (109). Neither program predicted a 5=splice site for the other species investigated either, suggesting a very weak 5= splice site for Mypt2 E24. This along with other features described below, likely accounts for the extremely tissue-restricted and phylogenetically limited splicing of this exon. Alternatively, given the internal PTC in Mypt2 E24, it is
conceivable that it could function as a terminal exon, in which case there would be no need for the 5=splice site, though there is no data to support this scenario at this time.
The COOH-terminal LZ motif coded for by skipping of Mypt2 E24 is highly conserved through fish (Fig. 3C,left). The alternative LZ motif encoded by E24 is 48 –52% conserved from human to fish and retains three of the four leucines of the alternative LZ motif (Fig. 3C,right).
Interestingly, a distinct 67 nt alternative exon has been identified in the chicken Mypt2 (63) located in the same intron and nearly 4.5 kb upstream of the sequence with homology to mammalian E24. This chicken Mypt2 alternative E24 has a PTC in the fourth codon, resulting in a COOH-terminal LZ⫺ variant (63). We could not identify with confidence sequences homologous to the distinct chicken Mypt2 E24 in the other species investigated. The chicken genomic sequence homolo-gous to mammalian Mypt2 E24 (Fig. 3B) has not been dem-onstrated to be a functional exon in chicken, though it could be that the proper tissues have not yet been examined, e.g., chicken skeletal muscle.
Sequence that is conserved between human and fish within the coding portion of the sequence of the Mypt2 E24 contains predicted binding sites for splicing factors 9G8 and Tra2(Fig. 3B) and an hnRNP A1 site which may act as a splicing silencer (12). Immediately upstream of the PTC is a conserved pre-dicted exonic identity element: AGGAGC (human-lizard)/ AGGACC (fish) (Fig. 3B, Supplemental Table S2). In contrast to Mypt1 E24, there is generally a lack of conserved SREs in the intronic flanking regions of Mypt2 E24: upstream of the 3= splice site is the pyrimidine-rich tract that is conserved as a feature but not at the level of individual nucleotides, while downstream of the 5=splice site there is a lack of conservation (Fig. 3B, Supplemental Table S2). An exception is the intronic region immediately flanking and including the 3= splice site, which is highly conserved and contains a predicted binding site for SRSF6 (SRp55) that spans the 3=splice site (Fig. 3B) and may inhibit recruitment of the U2 splicing factor. Additional conserved SREs were identified (Supplemental Table S2). The absence of conserved SREs near the 5= splice site and the nonconsensus 5= splice site itself is consistent with default skipping of the Mypt2 E24.
Complexity of the Mypt2 Locus
Mypt2, hsM21, smM21. PPP1R12B is a highly polymor-phic gene locus where a number of unique transcripts are generated by alternative splicing of exons (described above) and alternative transcriptional start sites (TSS). Unique TSS generate first exons (transcripts) unique to skeletal (Mypt2), cardiac (hsM21) and smooth (smM21) muscle (Fig. 4A) (1, 11, 30). Each of the annotated first exons of human Mypt2, hsM21, and smM21 are associated with indicators of tran-scriptional activity (H3K4Me1, H3K4Me3, and H3K27Ac), DNase hypersensitivity, and transcription factor binding as well as with TSS predictions (Fig. 4, B, C, E, red). This suggests a relatively unusual situation in which three loci within a single gene are under separate transcriptional control by the three muscle types. Interestingly, the TSS and first exon of hsM21 appears to differ among mammals. The first exon of human hsM21 is conserved in some species (e.g., rhesus, dog, elephant have 96 – 83% identity) but is
missing completely in the mouse and rat (Fig. 4C). Con-versely, the sequence of the annotated first exon of mouse hsM21 is conserved in humans (87.5% identity) and is located⬃11 kb downstream of the human hsM21 first exon (Fig. 4A, gray box). There is extensive conservation (⬎80%) between the human and mouse in the sequence immediately upstream (400 bases) of the sequence for the mouse hsM21 first exon. However, in the human this region lacks a predicted TSS based on human GenBank cDNAs (49, 50). There is also a lack of H3 modifications, DNase
hypersensitivity, and TF binding associated with transcrip-tional activity (Fig. 4D), which, in total, is consistent with different transcriptional start sites and first exons between mouse and human hsM21 with the caveat that these data were obtained from human cell lines. The mouse hsM21 first exon is highly conserved in the rat (95.4%), but only the 3=half of the exon is conserved in the other mammals (rhesus, dog, elephant, and opossum). Neither the human nor mouse hsM21 first exon se-quence is present in chicken or lower species raising the question of whether hsM21 is generated in these species.
E24-out E24-in chr1, +1: hg19 202,544,000 202,544,100 202,544,200 202,544,300 202,544,400 Mypt2 E24 Mammal Cons Vertebrate Cons
A
B
C
human mouse chicken lizard tetraodon medaka stickleback human mouse chicken lizard tetraodon medaka stickleback human mouse chicken lizard tetraodon medaka stickleback*
human mouse chicken lizard tetraodon medaka stickleback 1 2 3 4 5 6 7A
AG
G
A
CG
C
T
0 1 2 bits predicted 1C
T
2C3G
4C
A5T
6G
G
7C
8CGT
9 10G
A
11AG
12A
13A
14T15C
16A
G
17A18 19G
20G
C
0 1 2 bits 9G8/hnRNP A1 9G8 Tra2b 1 2 3 4 5 6 7 8 9G
GA
T
AG
AA
TC
G
A
0 1 2 bits 9G8 SRSF6 3’ splice signal 1 AC
A2 CT
3 GC
T
4TC5 G 6T
AG
7C
8A
9G
10 0 1 2 bits GA
11Fig. 3. Phylogenetic conservation of isoforms of Mypt2 generated by alternative splicing of Exon 24.A: conservation of the myosin phosphatase targeting subunit 2 (Mypt2) alternative exon and flanking region is shown by the phastCons track on the UCSC Genome Browser in the human hg19/GRCh37 genome release, as described in Fig. 1.B: mammalian (human, mouse) Mypt2 E24 and flanking regions are aligned to the homologous sequences in chicken, lizard, and fish PPP1R12B. Red lines and triangles highlight the known (mammalian) and predicted splice sites for E24. The red asterisk denotes the known (mammalian) and aligned stop codon. Conserved motifs were identified and analyzed for splicing cis-regulatory elements (seeMETHODS).C: diagrammed gene structure of the 3=
end of the Mypt2 gene indicates alternative splicing of E24 and the change of the open reading frame, in black. Amino acid sequence alignment of the COOH-terminus of Mypt2 demonstrates high conservation of the E24-out leucine zipper (LZ) motif (left). The E24-in LZ motif (right) is more variable in fish. Leucine residues of the LZ motifs are highlighted in gray.
In contrast the TSS and unique first exon of smM21 (11), located between exons 18 and 19 of Mypt2 are highly con-served in humans, chickens, and fishes (Fig. 4, A and E). Interestingly, the PPP1R12B ortholog in fish (tetraodon, medaka, and stickleback) appears to generate only the smM21 transcript; no genomic sequence with homology to mammalian Mypt2 spanning exons 1–18 is identified in the fish. This suggests that the original PPP1R12B gene may have only coded for the small M21 subunit, and that the NH2-terminal
ankyrin repeats and PP1c binding domains were later acquired through a recombination with another Mypt gene, most likely PPP1R12C given its closer phylogenetic relationship (Fig. 1B). Alternatively, the 5= end of the PPP1R12B gene could have been lost during fish evolution. Whereas the cause of this variability in the PPP1R12B gene structure during evolution is not defined, the variability itself is consistent with the difficulty in defining the function of MP (Mypt2) in striated muscle.
The complexity of the locus is compounded by the tissue-specific expression of these independent transcripts. Mypt2 is transcribed in the skeletal muscle, heart, and, to a lesser extent, brain (30); hsM21, as indicated by the name, has tissue-specific expression in cardiac muscle (1); and smM21 is expressed in smooth muscle (69, 88). Additionally, splicing of PPP1R12B E24 is highly regulated (1, 88). Inclusion of mammalian E24 in Mypt2 is highly restricted to skeletal muscle (unspecified type) in the context of the full-length Mypt2 transcript, with about half of the total transcript levels composed of the alternative isoform (1). Additionally, both the E24-in and E24-out iso-forms of hsM21 were cloned from human cardiac tissue (1), though there is no report of their relative proportions. Inclusion of the distinct avian PPP1R12B 67 nt E24 occurs in the context of smM21 and is also tissue specific, representing the primary isoform in the fast (phasic) gizzard smooth muscle (51, 63). In contrast inclusion of E24 (181 or 67 nt) in mammalian smooth 5kb 202,325,000 0 0 0 , 5 1 3 , 2 0 2 H3K4Me1 H3K4Me3 Mypt2 E1 H3K27Ac DNase Clusters TF ChIP Mammal Cons Vert Cons
B
Lizard 0 0 0 , 5 3 4 , 2 0 2 0 0 0 , 5 2 4 , 2 0 2 5kb H3K4Me1 H3K4Me3 hsM21 E1 H3K27Ac DNase Clusters TF ChIP Mammal Cons Vert Cons Rhesus Mouse Rat Dog Elephant Opossum ChickenC
5kb 202,450,000 0 0 0 , 0 4 4 , 2 0 2 H3K4Me1 H3K4Me3 mouse hsM21 E1 H3K27Ac DNase Clusters TF ChIP Mammal Cons Vert ConsD
5kb 202,500,000 202,515,000 H3K4Me1 H3K4Me3 smM21 E1 H3K27Ac DNase Clusters TF ChIP Mammal Cons Vert ConsE
5 2 4 2 3 2 9 1 8 1 6 1 4 1 3 1 2 1 3...12 21ATG Mypt2 ATG hsM21 ATG smM21 TAG E24-in
TAG E24-out
A
) E ( ) D ( ) C ( ) B ( TSS Mypt2 TSS hsM21 TSS smM21 / Fish PPP1R12B mouse hsM21 E1Fig. 4. Complexity of the Mypt2 (PPP1R12B) locus.A: protein phosphatase 1 regulatory subunit 12B (PPP1R12B) locus hosts independent transcriptional start sites (TSS) for Mypt2 (982 aa), heart-specific M21 (hsM21) (208 aa), and smooth muscle M21 (smM21) (186 aa). The first exons and start codons (ATG) for each transcript are highlighted and color coded in this diagram that uses the human PPP1R12B as the template (red: Mypt2; blue: hsM21; green: smM21). The alternate stop codons (TAG) are also diagrammed. The region corresponding to the first exon of the mouse hsM21 is depicted by a gray box. Experimental evidence for independent transcriptional regulation (regions of histone 3 modification, DNase hypersensitivity, TF ChIP) is shown for the first exons of human Mypt2(B) hsM21 (C), and smM21 (E). SwitchGear predicted TSSs are depicted as red lines. The mammalian and vertebrate conservation is also shown.D: experimental evidence for transcriptional regulation of the first exon of mouse hsM21 and conservation are shown.
muscle smM21 transcripts is not identified in published studies (63) nor public gene expression datasets. More thorough in-vestigations of the pattern of splicing of Mypt2 E24 are required to define its tissue specificity, potential coupling to gene transcription, and phylogenetic conservation.
MP Inhibitory Subunits
Four family members of the inhibitory subunit of myosin phosphatase (PPP1R14A-D) have been identified with little variation within each transcript (21). Exon 2 of CPI-17 (PPP1R14A) has been reported as alternative in human aorta (118), but our surveys did not uncover an exon skipped variant in a survey of rat smooth muscle tissue (data not shown) and there are no other reports of this variant in the databases. Alternative translational start sites have been proposed for human and mouse PHI (⫺1,2) (PPP1R14B) (22), but using the current mouse and human genome sequence the proposed PHI-2 AUG sequence 167 nt and 154 nt upstream of the well-validated start codons of mouse and human PHI-1, re-spectively,1) lack Kozak consensus sequence for translational initiation and 2) would change the reading frame and cause premature termination after 24 amino acids, casting doubt on this as a bona fide translational variant.
The diversity in the inhibitory subunits arises by their regulated transcription, yet the expressional and functional relationships between the inhibitory subunit family members are not well defined. The initial reports of CPI-17 (PPP1R14A), the first identified family member, demonstrated high levels of expres-sion specific to smooth muscle (23). This finding is confirmed in public databases of human and mouse RNA-Seq and mi-croarray tissue surveys (85, 88), with high levels of CPI-17 transcript in the aorta, bladder, lung, and prostate and much lower levels in striated muscle and nonmuscle cells, mirroring Mypt1 expression. In contrast, PHI (PPP1R14B) expression is fairly ubiquitous with published immunoblot evidence of pro-tein expression (22) and ubiquitous expression in RNA-Seq data in general agreement (85, 88). The expression of the other inhibitory subunit family members KEPI (PPP1R14C) and gastric brain phosphatase inhibitor (GBPI) (PPP1R14D) is less pervasive and less robust. Published studies have characterized KEPI expression as most robust in cardiac tissue (60) while GBPI expression is primarily limited to the colon (59).
There is evidence both for (18) and against (81) redundancy between the two main inhibitory subunits expressed in smooth muscle, PPP1R14A and B, yet there are no mouse knockout experiments to define the roles of the subunits. Our unpub-lished data from Deep RNA sequencing indicates that CPI-17 (PPP1R14A) transcripts are approximately two- to ninefold higher than PHI-1 in vascular smooth muscle (unpublished data, RP Dippold and SA Fisher). Consistent with restricted expression and specialized function of PPP1R14A, the region upstream of the PPP1R14A TSS lacks TATA and CAAT boxes, has little phylogenetic conservation, and lacks indica-tors of transcriptional activity in cell lines (Fig. 5A). A limited region of 188 bases ⬃300 –500 bases upstream of the TSS could be aligned between human and mouse and putative TFBS identified, including a GC box that was previously identified and shown to function as a minimal promoter in vitro [(52) and Supplemental Table S3].
We used a number of criteria in an attempt to computation-ally identify potential PPP1R14A (CPI-17) transcriptional en-hancers (seeMETHODS) (36, 111). Regions within the first intron
immediately upstream of exon 2 and within the third intron are well conserved suggesting possible regulatory functions (Fig. 5A). These regions were hypersensitive to DNAse and con-tained histone modifications consistent with enhancer activity. Algorithms predicted a number of binding motifs for TFs involved in smooth muscle phenotypic determination (re-viewed in Ref. 55), including Enhancer box (E-box), cAMP response element-binding protein (CREB), nuclear factor of activated T-cells (NFAT), and peroxisome proliferator-acti-vated receptor-␥(PPAR␥) but notably no CARG motifs for the binding of serum response factor (SRF) and myocardin, a well-validated smooth muscle cis-regulatory motif (reviewed in Ref. 82). The absence of indicators of transcriptional activity near the TSS likely reflects the tissue-specific transcription of PPP1R14A and its low expression in the various cell lines in which these assays are performed.
In contrast to PPP1R14A, PPP1R14B appears to have con-siderably more transcriptional activity in cell lines as is sug-gested by the histone 3 modifications, DNase hypersensitivity, and TF ChIP (Fig. 5B), likely indicative of its un-restricted expression. The intronic and upstream regions of PPP1R14B are more highly conserved, allowing for a more extensive report of conserved TFBS. Potential conserved regulatory elements include a muscle-specific TATA (mTATA) box (gctggcccTTTAAGggg)-SRF combination 44 bp upstream of the TSS, an AP2 site within 20 bp of the SRF site, multiple SP1 sites, and an Ebox site in the conserved region proximal to the TSS (Fig. 5B). The first intron of PPP1R14B contains many conserved, high-scoring putative TFBS (Fig. 5, B2, Supple-mental Table S4). As with the upstream promoter region, several of the putative TFBS in the first intron are involved with muscle-specific gene expression, muscle differentiation, homeostasis, and growth such as Ebox, NFAT, CREB, and Forkhead box protein O1 (FOXO1) (reviewed in Ref. 5). This computational prediction of transcriptional control of the MP inhibitory subunits provides a foundation for experimental testing of their functional importance in different cell types.
DISCUSSION
The control of muscle function by protein phosphorylation reflecting the regulated activities of Ser-Thr Type 1 phospha-tases and kinases is pervasive and also muscle type specific. Yet there remains limited understanding of the diversity within the components of the signaling pathway and its effect on the control of muscle function. Here we focused on the myosin phosphatase and analyzed public databases to define modes of diversity and the regulation of the variability of the MP subunits. Considerable diversity is present within its regulatory subunits, which reflects evolutionary genomic diversification as a whole and includes increases in the number of regulatory (inhibitory) gene family members multiplied by greatly in-creasing combinations of alternative transcriptional start sites and exon splicing that vastly increase the number of unique transcripts generated from each gene locus.
The completion of sequencing of many genomes facilitated a phylogenetic analysis of Mypt1 E24 splice variants. The skipping of Mypt1 E24, the evolutionary and tissue default,
codes for a COOH-terminal LZ motif, a highly conserved feature of Mypt family members (Mypt1, Mypt2, and MBS85; reviewed in Ref. 34), consistent with its proposed role in LZ-mediated heterodimerization with cGK1␣and NO/cGMP-dependent activation of MP (42, 51, 101) (57). The “LZ,” in which a leucine (or isoleucine) residue is present at every seventh amino acid in the context of an a-helical coiled-coil domain, was originally described in what is now termed the B-ZIP family of transcription factors (56). In this large family, high throughput and computational studies suggest that speci-ficity in LZ-mediated hetero- and homodimerizations produce great diversity and specificity in the transcriptional output (90, 106). There is some evidence for similar specificity and diver-sity in LZ-mediated interactions in the regulation of myosin phosphatase and other contractile proteins controlling muscle function (reviewed in Refs. 15 and 39). Mypt1 and Mypt2 have alternative COOH-termini generated by alternative splicing of E24 that abolishes or creates an alternative LZ motif, respec-tively. The Mypt1 E24 sequence is highly conserved as an alternative exon from fish to mammals, but not present in the
ancestral ortholog in flies and worms, and is also absent from some vertebrate classes such as amphibians. This suggests that this alternative exon emerged during evolution and was under strong selection pressure to be retained. We propose that it arose as a mechanism to suppress NO/cGMP regulation of MP activity in phasic smooth muscle tissues. A number of studies have shown that NO or its second messenger cGMP are unable to activate MP as a means for relaxation of prototypical phasic smooth muscle such as the rat portal vein (26, 83) and chicken gizzard (51, 86). MP expression and activity is severalfold higher in phasic versus tonic smooth muscle (31), all support-ing the hypothesis that higher basal, yet unregulated MP activity, at least with respect to NO/cGMP signaling, is re-quired for cycling of phasic smooth muscle contraction and relaxation. This hypothesis could be tested by deletion of E24 in the mouse converting all smooth muscle tissues to the E24⫺ (LZ⫹) isoform.
There has been less study of the expression pattern of the Mypt2 E24 splice variants coding for COOH-terminal LZ variants. The few studies that have examined this describe the chr19: 38,742,000 2 kb 38,750,000 H3K4Me1 H3K4Me3 PPP1R14A H3K27Ac DNase Clusters TF ChIP Mammal Cons Vert Cons chr11: H3K4Me1 H3K4Me3 PPP1R14B H3K27Ac DNase Clusters TF ChIP Mammal Cons Vert Cons 64,012,000 2 kb 64,017,000 1 2 1 2
B
A
1 2 E1 AP2 KROX ETS TFIIA CACD MTATA ETF SP1(x2) GC SRF SP1 GC SP1 NKX2.5 GC Ebox Ebox NMYC ETS AHR KROX YY1 EGR SP1 AP2 E2F AP2 NFY ACCAT ETSNFAT 200 bp 64,015,645 64,014,343 E2 E1 TBX5 SP1 Ebox EboxAP4 NFAT GATA6 SF1 AP2 SF1 T3R NFkB ETS NRF2 ELK1 AR Ebox AP2 SMAD3 Ebox ETS 200 bp Ebox AP4 SP1(x2)E2F1 Ebox PEA3 NKX2.5VDR CREB ETS1 NFAT SRY SOX P53 SP1 AP4 SP1 TEF1 Ebox MAF SP1 AP2 AP1 CREB T3R AP2 CREB AP1 ER T3R EGR E2F1 SP1 E2F1 64,012,700 64,013,913 Stat EBF T3R CP2 SP1 ETF UF1H3b SP1 EGR1 SMAD T3R Stat Stat CREB SMAD TEF1 SP1 AP2 AP2 Ebox MEIS1 MZF1 CREB AP1 Musc Ini Ebox SMAD Ebox NFMEU1 HEB AP4 LBP1 LFA1 100 bp 0 0 1 , 4 4 7 , 8 3 5 3 7 , 3 4 7 , 8 3 100 bp TFIII MAZ MZF KROX WT1 SP1 Ebox ETS GC GATA4 MAF NERF P300 ETS PEA3 NKX2.5 TATA NFAT IRF PPARG HNF4 EBF HNF4 CREB COUP ATF E3 E4F1 E4F1 38,742,995 38,742,430 1 2Fig. 5. Analysis of CPI-17 (PPP1R14A) and PHI-1 (PPP1R14B) noncoding sequence for potential transcriptional regulatory activity.A: protein phosphatase 1 regulatory subunit 14A (PPP1R14A). Portions of noncoding sequence in introns 1 and 3 (red boxes 1 and 2) are well conserved between human and mouse and have other indicators suggestive of transcriptional enhancer activity (H3 modification, DNase hypersensitivity, and TF ChIP). The transcription factor binding sites (TFBS) predicted in each of these blocks of sequence are shown. The TFBS in black are predicted using the rVista “optimized for function” approach to reduce false positives (seeMETHODS). The TFBS in gray have a singular cutoff of 0.85 similarity to the TRANSFAC positional weighted matrix (PWM) and are of interest to muscle gene regulation.B: PPP1R14B. noncoding sequence is well conserved immediately upstream of the TSS and in intron 1 (red boxes 1 and 2) and have other features suggestive of transcriptional promoter and enhancer activity, respectively. The TFBS predicted in each of these blocks of sequence are shown. The full compilation of TFBS for the two cutoffs can be found in Supplemental Tables S3 and S4.
sample as skeletal muscle, leaving open the possibility that the expression of the variants may vary by striated muscle type (fast, slow), species, or developmental age. Interestingly, the fish Mypt2 homologue only codes for the small (M21) subunit, suggesting that either1) MP activity is not required for striated muscle function in fish or2) this function is served by another Mypt family member. LZ-mediated dimerization partners of each Mypt2 LZ variant have not been determined, though given the strong similarity of the E24⫺encoded LZ to Mypt1 (and MBS85) it seems likely that it would also bind PKG1␣. Both E24⫺ and E24⫹-encoded Mypt2 LZ motifs are slightly basic and have similar charge profiles. However, the E24⫹ encoded LZ motif is uniquely followed by a COOH-terminal acidic tail of 11 residues that could modulate interactions with regulatory proteins. Entirely missing from the models of LZ motifs and MP protein interactions is consideration of the small (M21) subunit of MP, which has yet to be well characterized in terms of tissue-specific expression of isoforms and function. Finally, it is worth noting that the upstream regulatory kinase PKG1 also has two evolutionarily conserved isoforms contain-ing alternative NH2-terminal LZ motifs (␣,) generated from
alternative transcriptional start sites (75). The individual LZ motifs of the two isoforms are thought to create specificity in their substrates though there remains limited data in this regard (reviewed in Refs. 8 and 39). The current study describing the tissue-specific and phylogenetic patterns of expression of the MP LZ containing subunits provides a foundation for experi-mental testing of the role of PKG1 activation of MP in muscle-type specific control of function in appropriate model organisms.
Portions of the intronic sequence flanking Mypt1 E24 are also highly conserved, consistent with a prior genome-wide evolutionary study of alternative splicing that found evolution-ary conservation of alternative exons was associated with conservation of flanking intronic regulatory sequences (67). Aside from the splice site junction sequences, the most invari-ant sequence is the hexanucleotide 5=-TCTGAA-3=located just upstream of the AG of the 3= splice site. This sequence was computationally predicted to be an exonic splicing enhancer and exonic identity element (25, 119). Exonic elements of this nature are thought to be necessary for proper exon identifica-tion and splicing (4). However, when found in intronic regions flanking exons, they can act as splicing repressors (48, 66, 119). The proximity of this element to the E24 3=splice site would predict that it would suppress splicing of the exon by blocking recruitment of U2 to this site. Its function as a cis-regulator of E24 splicing remains to be tested, which given its conservation could be performed in any model system and most expeditiously in the zebrafish. The high conservation of Mypt1 E24 sequence among higher vertebrates (mammals, birds, reptiles: 26/31 nt identity) is less present in the fish. The exonic cis-element (GCAAGAGU) that binds the splicing factor Tra2and enhances splicing of Mypt1 E24 (29, 97) is absent. Whether this results in less efficient tissue-specific splicing of E24 in fish or reflects different control mechanisms requires further study. Predicted binding sites for other classic Ser/Arg-rich (SR) splicing factors [SRSF2 (SRp30b) and SRSF6 (SRp55) (92)] are also highly evolutionarily conserved and could be involved in the regulated splicing of this exon. Overall, there remains limited understanding of the regulation of alternative splicing in the generation of smooth muscle
phenotypic diversity and somewhat more understanding of this topic in striated muscle (reviewed in Refs. 27, 32, and 46). The highly programmed and tissue-specific nature of Mypt1 and Mypt2 E24 splicing make them good candidates for model exon approaches to this problem.
The four PPP1R14 family members have much less complex gene structures with little variability within individual genes, not surprising given their small size and few (4 –5) exons. There is a single and well-validated report of a splice variant of PPP1R14A (CPI-17) in human aortic tissue in which skipping of exon 2 deletes a segment of the PP1 inhibitory domain (118). However, alternative splicing of this highly conserved exon in other species is not present in the queried databases such that this variation may be unique to humans. Rather, the diversity in the inhibitory subunit seems to derive from the variability among the family members and the highly regulated and tissue-specific expression of these genes, though it should be acknowledged that the function and relationships between the family members in vivo have not yet been defined through gene knock-outs. Expression of CPI-17 mRNA and protein is highly tissue-specific, being greatly enriched in smooth muscle with some variation between muscle types (117), and dynam-ically altered in disease (41, 71, 73, 74, 93). Little is known about its transcriptional regulation in these contexts. An up-stream minimal promoter was identified (52), but there is little conservation of this upstream region between species, and no CAAT or TATA boxes, all consistent with a highly regulated transcript. We found highly conserved noncoding sequence in introns 2 and 3 that are predicted to contain a number of transcriptional cis-regulatory elements. Some transcription fac-tors that bind these predicted elements, such as NFAT and PPAR␥ (5, 91), mediate the slow muscle gene program and regulate responses to external signals, as do CREB, Stat, SMAD, and Ets (reviewed in Ref. 5). Definition of these conserved and predicted transcriptional enhancer domains will require functional testing; not all regulatory sequences are conserved, and conservation of noncoding and putative regu-latory sequence provides an increased likelihood but not cer-tainty that they would function as such. In contrast PHI has the features of a less tightly regulated gene, with a highly con-served upstream sequence that in cell lines has histone marks and DNAse hypersensitivity indicative of an active promoter.
Perspectives and Signficance
The phosphorylation and dephosphorylation of myosin is the primary means by which force is controlled in smooth muscle and is thought to provide a modulatory role in striated muscle. In this study we have used computational analyses of large publicly available databases to describe the diversity in MP subunits. This more comprehensive analysis has revealed a number of features of the MP subunits that are predicted to underlie the functional significance and expressional regulation of this diversity. Of particular note are1) the deeply conserved alternative splicing of Mypt1 E24, putative upstream splicing regulatory element, and PTC, 2) the substantial phylogenetic variability at the Mypt2 (PPP1R12B) locus with a variety of tissue-specific transcripts and alternative splicing that matches its uncertain role in striated muscle function, and3) the phylogenetic expansion of the inhibitory subunit (PPP1R14A-D) gene family with little intragenic diversity, and difference in structure of
putative promoter-enhancers between the highly (CPI-17) and less highly (PHI-1) regulated family members. This comprehensive phylogenetic analysis of MP subunit diversity will enable the optimal selection of model organisms for testing hypotheses as to the regulation and function of subunit isoforms in determining specificity in the control of signaling pathways that regulate muscle function.
GRANTS
This work was supported by National Institutes of Health Grants HL-66171 to S. A. Fisher and T32 AR007592 to R. P. Dippold.
DISCLOSURES
No conflicts of interest, financial or otherwise, are declared by the author(s).
AUTHOR CONTRIBUTIONS
Author contributions: R.P.D. and S.A.F. conception and design of research; R.P.D. performed experiments; R.P.D. and S.A.F. analyzed data; R.P.D. and S.A.F. interpreted results of experiments; R.P.D. prepared figures; R.P.D. drafted manuscript; R.P.D. and S.A.F. edited and revised manuscript; R.P.D. and S.A.F. approved final version of manuscript.
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