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Selective activation of the mitogen activated protein kinase subgroups c Jun NH2 terminal kinase and p38 by IL 1 and TNF in human articular chondrocytes

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Figure 1. ERK are ma-Whole cell lysates were subjected to Western blotting with antibody (4G10) to phosphotyrosine (antibody that recog-nizes both, ERK-1 and ERK-2 (jor tyrosine-phosphory-lated proteins in IL-1 stimulated chondro-cytes
Figure 2. Tyrosine
Figure 4. Expression of p38 in chondrocytes. Lysates from unstimu-Western blotting was performed with antibody to COOH terminus (CT, first 2 lanes) or with antibody to NHlated chondrocytes were subjected to Western blotting without im-munoprecipitation (no
Figure 7. Second messenger agonists and MAP kinase activation. mM), PMA (10 ng/ml), A23187 (2 Chondrocytes were stimulated with IL-1� (1 ng/ml), db-cAMP (1 �M), or PMA plus A23187 for 30 min

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