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New Real Time PCR Assay Using Allelic Discrimination for Detection and Differentiation of Equine Herpesvirus 1 Strains with A2254 and G2254 Polymorphisms

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Academic year: 2020

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Figure

TABLE 1 EHV-1 ORF30 specific primer/probe sets used in E1 and E2 allelic-discrimination rPCR assays
FIG 21 and E. The average C values for each dilution were generated from two runs of eachassay and then used to plot a linear trend line
TABLE 2 E1 and E2 real-time PCR results
TABLE 4 Sequence analysis of molecular clones derived from thesamples that had dual genotypes (A2254 and G2254) in the E1 assay

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