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Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling

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Figure

Fig. 1. Characterization of Med23 KO ESCs. (A) Wild-type(WT) and KO ESCs were plated at clonal density with feedercells for 2 days and then stained for alkaline phosphataseactivity (red)
Fig. 2. Enhanced neuroectoderm specificationin spontaneous differentiation of Med23 KOESCs
Fig. 3. Med23 deletion promotes neural differentiation inN2B27 culture. (A) ESCs were cultured under monolayerdifferentiation conditions in the presence of N2B27.Expression levels of marker genes for ESC self-renewaland neural precursor cells were measured
Fig. 4. Enhanced neural differentiation is reversed byre-expression of Med23. (A) Immunoblot of MED23 inwild-type, Med23 KO and Med23 KO+exogenous Med23ESCs
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