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Development and Clinical Evaluation of an Internally Controlled, Single Tube Multiplex Real Time PCR Assay for Detection of Legionella pneumophila and Other Legionella Species

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Figure

TABLE 1. Primers and probes for PCR amplification of Legionella spp. (16S rRNA gene) and L
FIG. 1. (A) Relative fluorescence units obtained for a 10-fold dilution series of L. pneumophilaslope of the line gives a value for PCR efficiency; in this case it is 100%
TABLE 3. Culture and real-time PCR results for clinical samplesa

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