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Different genome stability proteins underpin primed and naïve adaptation in E coli CRISPR Cas immunity

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Academic year: 2020

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Figure

Figure 1.RecG, PriA and DNA polymerase I (PolA) are needed3) and tofor primed adaptation
Figure 2.(Agarose gels summarizing effects on primed adaptation in thetion strain when expressing mutant RecG proteins from a plasmid (lanes1–5), or RNaseHI inducibly expressed from the chromosome (Lanes 6 and7)
Figure 3. DNA binding and catalysis bypre-mixing of Cas1 with PriA, as indicated above the panel, results in loss of detectable Cas1 nicking fork-1
Figure 4.Model proposing different requirements for DNA capture inactivities, discussed in the text, until removed by RecG helicase activitythat remodels forks and removes R-loops

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