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ciliaFA : a research tool for automated, high throughput measurement of ciliary beat frequency using freely available software

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Figure 1 (A) Diagrammatic view of the change in light intensity surrounding motile cilia constructed using the Volume Viewer pluginfor ImageJ
Figure 2 Data analysis of one. AVI file of ependymal cilia before (A), and 15 minutes (B) after the addition of the bacterial toxin, pneumolysin.Images show (i) the area of brain tissue investigated, (ii) the regions of interest (ROI) used for analysis (color coding shows the intensity of the ciliarybeat frequency (CBF) for each ROI: the darker the displayed color, the higher the CBF) and (iii) a histogram of the CBF of all regions of interest.
Figure 3 Linear regression of the mean ciliary beat frequency (CBF) measurements by conventional frame by frame counting ofindividual ciliary beat cycles (direct counting method) compared with the ciliaFA system (A) using ependymal cilia (n = 100) and (B)respiratory cilia (n = 100) at 37°C.
Table 1 The ependymal ciliary beat frequency (CBF) determined by two observers using high-speed digital imagingand the ciliaFA software

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