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Short term ex vivo expansion of circulating head and neck tumour cells

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Figure

Figure 1: Immunofluorescent staining of CTCs from RosetteSep™ enrichred head and neck cancer patient blood samples (Row 1: Pt #10, Row 2: Pt #17), stained with an antibody cocktail (Cellsearch®: anti-Cytokeratin 8/18/19, DAPI, anti-CD45) and anti-EGFR
Table 1: Clinical details, CTC counts and culture notes of short term culture positive samples
Figure 2: Short term CTC cultures in 2D (MSK media, day 7, 2% O2). Row (A) 96 well standard microplate, Row (b) Corning® Spheroid Microplates (ThermoScientific, USA)
Figure 3: CTC count vs short term culture success. P = 0.0002; Mann-Whitney test. Error bars represent standard deviation.
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