Caries Activity Tests

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CARIES ACTIVITY TESTS

Dr

Dr sha

shabee

beel

l pn

pn

www.hi-dentfinishingschool.blogspot.com www.hi-dentfinishingschool.blogspot.com http://apexiondental.com/

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CARIES ACTIVITY TESTS

DENTAL CARIES DENTAL CARIES

IItt iiss aann iirrrreevveerrssiibbllee p

prrooggrreessssiivvee ddiisseeaassee oof f m

muullttiiffaaccttoorriiaall iinn nnaattuurree aaffffeeccttiinngg tthhee ccaallcciiffiieedd ttiissssuueess ooff tthhee tteeeetthh cchhaarraacctteerriizzeedd bbyy d

deemmiinneerraalliizzaattiioonn oof f iinnoorrggaanniicc ppoorrttiioonn && d

deessttrruuccttiioonn ooff oorrggaanniicc p

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CARIES ACTIVITY

Re

Refe

fers t

rs to the i

o the incr

ncrem

ement

ent of ac

of acti

tive l

ve lesi

esion (n

on (new an

ew and

d

recurrent lesions) over a stated period of time

Car

Caries

ies act

activi

ivity

ty is a

is a mea

measure

sure of sp

of speed

eed of pr

of progre

ogressi

ssion

on

of a carious lesion.

CARIES SUSCEPTIBILITY

Ref

Refers

ers to t

to the i

he inher

nherent

ent ten

tendenc

dency

y of t

of the ho

he host &

st & tar

target

get

tissue, the tooth to be affected by the cario

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REQUIREMENTS OF CARIES ACTIVITY

TESTS

Te

Test st shshouould ld be rbe repeproroduduciciblble e & va& valilidd The

There sre shoulhould be d be good good corrcorrelaelatiotion ben betwetween en the cthe cariaries aes actictivitvityy scores & actual caries development

scores & actual caries development S

Shhoouulld d bbe e ssiimmppllee Res

Resultults ss shoulhould be d be obtaobtained ined rapirapidlydly, wi, withithin houn hours rs or for few daew daysys Sho

Should uld have have meameasuresuremenment ot of mef mechanchanismisms ins involvolved ved in cin cariarieses process

process Sho

Should uld be ibe inexpnexpensiensive, ve, non-non-invinvasiasive ve & ap& appliplicablcable e to ato anyny clinical setting

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CARIES ACTIVITY

TESTS:

1

1.. LLacacttobobaacciilllluus s ccoolloonny y ccoouunnt t tteesstt S

Saalliivva ia is cs coolllleecctteed bd by cy chheewwiinng pg paarraaffffiinn before breakfast

before breakfast T

Thhe e ssppeecciimmeen n iis s vviiggoorroouusslly y sshhaakkeen n aanndd after that 0.1 cc of sample is withdrawn after that 0.1 cc of sample is withdrawn D

Diilluutte e aannd d uunnddiilluutteed d ssaammppllees s aarre e tthheenn spread evenly over a rogosa¶s SL agar spread evenly over a rogosa¶s SL agar plate

plate T

Thhe e ppllaatte e iis s iinnccuubbaatteed d ffoor r 4 4 ddaayys & s & nnoo. . oof f lactobacillus colonies that developed are lactobacillus colonies that developed are counted

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No of organisms

1-1000

1000-5000

5000-10,000

More than 10,000

Symbolic

designation

+

++

+++/++++

Degree of caries

activity suggested

Little or none

Slight

Moderate

Marked

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SNYDER TEST SNYDER TEST

Th

This is tetest mst meaeasusureres ths the ae abibilility oty of saf salilivavaryry

microorganisms to form organic acid from a microorganisms to form organic acid from a carbohydrate medium.

carbohydrate medium. The

The clclassassicical al forformumula la of Sof Snynyderder¶s ¶s agaagar per per lr lititrere of purified water is

of purified water is

pancreatic

pancreatic digest/ digest/ casein casein -13.5 -13.5 gmgm y

yeeaasst t eexxttrraacctt --66..5 5 ggmm d

deexxttrroossee --220 0 ggmm ssooddiiuum m cchhlloorriiddee --5 5 ggmm aaggaarr --1166 gmgm B

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24

24 hrs hrs 48 48 hrs hrs 72hrs72hrs C

Coolloor r : : yyeelllloow w yyeellllooww yyeellllooww Caries

Caries activity: activity: marked marked definite definite limitedlimited

Color

Color : : green green green green greengreen Caries

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ALBEN¶S TEST ALBEN¶S TEST

IIt it is a s a mmododiifified ed SSnynydeder tr tesestt At

At the the timtime of e of testest 5 mt 5 ml, tl, tube ube of sof semiemisolsolid aid agar gar is is remremovedoved from refrigerator but is not heated

from refrigerator but is not heated The

The patpatienient it is askes asked to sd to spit pit unstunstimimulaulated ted salsaliva diva direirectlctly in ty in too the tube. Tube is incubated for 4 days.

the tube. Tube is incubated for 4 days. SWAB TEST

SWAB TEST Ad

Advanvantatage ge is is no no colcollelectctioion n of of salsaliviva ia is ns neceecessssararyy Val

Valuabluable ie in evan evalualuatinting cg cariaries aces activtivity ity in vein very yry young coung chilhildrendren Pr

Prininciciplple e is is sasame me as as SnSnyydeder tr tesestt The

The oraoral fll flora iora is sams samplepled by d by swaswabbibbing tng the bhe buccauccal sl surfurface ace of of tooth with cotton.

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REDUCTASE TEST REDUCTASE TEST

Thi

This tes test mst measeasures ures the athe actictivitvity of ry of reduceductastase ee enzynzyme prme presenesent it inn salivary bacteria

salivary bacteria The

The samsample ple is mis mixed ixed witwith fih fixed xed amoamount unt of dof diaziazo-ro-resoresorcincinolol Th

The che changange ie in coln color aor aftfter 1er 15 mi5 min is tan is taken ken as a mas a meaeasursure of e of caries activity

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ccoolloorr TTiimme e ssccoorree CCaarriiees s aaccttiivviittyy Blue Blue Orchid Orchid Red Red Red Red pink pink 15min 1 15min 1 15 15 min min 22 15 15 min min 33 Immediately 4 Immediately 4 Immediately 5 Immediately 5 Non conductive Non conductive Slightly conductive Slightly conductive Moderately Moderately conductive conductive Highly conductive Highly conductive Extremely conductive Extremely conductive

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ENAMEL SOLUBILITY

TEST

It

It is is babasesed od on tn the he fafact ct ththat at whwhen en glglucucososee is added to

is added to saliva containing powderedsaliva containing powdered enamel, organic acids are formed

enamel, organic acids are formed Org

Organanic ic acacid id dedecacalclcififieies ts the he enenamamelel,, resulti

resulting in an ng in an increase in the amount of increase in the amount of soluble calcium

soluble calcium Th

The e exextetend nd of of inincrcreaease se of of cacalclciuium m is is aa direct measure of caries activity

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SALIVA FLOW TEST

Fl

Flow row rate ate is deis detertermimined ned by coby collellecticting ng parparaffaffin in stistimulmulateatedd saliva in a test tube over 5 min

saliva in a test tube over 5 min Sev

Severelerely dy decrecreaseeased fd flow low is ris relaelated ted to cato carieries sus suscesceptiptibilbilityity As

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P

ATIENT EDUCATION WITH METHYL RED

A si

A simplmple and e and effeffectective ive tectechniqhnique ue that that may may be of be of assassististance ance inin educating child patient to the problem of dental caries control educating child patient to the problem of dental caries control involves the use of aqueous solution of methyl red

involves the use of aqueous solution of methyl red Ind

Indicaicator tor dydye chae changes nges colcolour our in thin the pH e pH rangrange fe from rom 6.3(6.3(disdistintinctct ye

yellollow) w) ±± 4.24.2(re(red)d) Aqu

Aqueous eous metmethyl hyl red red is tis then hen applapplied ied to tto the she surfurface ace of thof the toe toothoth with dropper

with dropper Red

Red colcolour our is deis develveloped oped in tin the ahe area rea of pof plaqlaque ue accuaccumulmulatiationon Thi

This is is is internterpretpreted ed to pato patietient nt as evias evidencdence e of conof contintinuous uous aciacidd formation

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