PperCHIP. High-Content Peptide Microarrays. Epitope Mapping & Serum Profiling Services

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High Content Peptide Microarrays

Epitope Mapping • Serum Profiling • Peptide Engineering

PEPperCHIP

®

Get access to custom and standard peptide microarrays with up to 9,000 features per chip in a uniquely flexible and cost-effective manner.

PEPperMAP

®

Benefit from our new peptide microarray platform and let PEPperPRINT's experts get the results for you.

High Content Peptide Microarrays

Epitope Mapping • Serum Profiling • Peptide Engineering

PEPperCHIP

®

Get access to custom and standard peptide microarrays with up to 9,000 features per chip in a uniquely flexible and cost-effective manner.

PEPperMAP

®

Benefit from our new peptide microarray platform and let PEPperPRINT's experts get the results for you.

PEPperPRINT GmbH Rischerstraße 12 D-69123 Heidelberg Fon: +49 (0) 62 21 72 64 489 Fax: +49 (0) 62 21 72 64 475 pepperchip@pepperprint.com www.pepperprint.com

PePperChiP

®

High-Content Peptide Microarrays

PepSlide

®

Analyzer

Custom Peptide Synthesis

PePperMAP

®

Epitope Mapping & Serum Profiling Services

PepSlide

®

Analyzer Specifications

Quantify and analyze your peptide microarray data with our intuitive and easy-to-use software solution:

• use microarray scanners‘ TIFF images and load design data from GAL files or PepSlide® PSF files

• efficient tools for image processing, array alignment and spot finding

• perfectly adapted to the PEPperCHIP® platform

• full compatibility with Excel

• recognition of custom spot sizes and morphologies • state of the art background correction

• cost-effective alternative to GenePix® Pro

Peptide Specifications

• HPLC purified custom peptides (95% purity on demand)

• 1 mg (€ 11/amino acid), 5 mg (€ 22/amino acid) and 10 mg (€ 33/amino acid) batches

• 16, 96 and 384-well small scale peptide libraries • linear and cyclic peptides

• various modifications available (phosphorylation, PEGylation, biotinylation, acetylation, methylation etc.) • useful for competition and enzymatic assays, peptide

immobilization and peptide array spotting

Please inquire for other peptide purities, quantities and modifications.

(2)

PePperChiP

®

PEPperCHIP

®

Specifications

• custom and off-the-shelf peptide microarrays • up to 9,000 features per chip

• thorough customization covering any protein or peptide collection

• fully adjusted to your research

• standard slide formats with wide chip reader compatibility

• GenePix® Pro and PepSlide® Analyzer support

• most cost effective solutions

PEPperMAP

®

Specifications

Benefit from PEPperPRINT’s state-of-the-art peptide microarray platform PEPperCHIP® without

any need for time, manpower or technical infra-structure.

Just send us your antibody or serum sample and let PEPperPRINT’s experts get the results for you. We are pleased to provide you with standard epitope mapping services and custom serum profiling solutions for your research.

PEPperCHIP® - PEPperPRINT’s new peptide microarray platform with custom and standard contents

PEPperMAP® - The most convenient way to benefit from PEPperPRINT’s new peptide microarray platform.

Custom peptide microarrays with more than 8,000 features for the discovery of serum signatures (left) and full permutation scans of a known antibody epitope (right)

Epitope mapping of a polyclonal antibody against two antigens as overlapping peptides in double spots (red; stained control peptides frame the antigen-derived peptides)

PEPperCHIP

®

Accessories

• PEPperCHIP® Incubation Trays with 3/1, 3/2, 3/3, 3/4, 3/5, 3/16 and 3/(3,4,5) well formats

3

3 one-by-one assays with multiple array copies

3

3 minimal sample consumption

3

3 enables shaking incubation

• PEPperCHIP® Staining Kit with labeled anti-HA and anti-Flag

antibodies for control assays

• PepSlide® Analyzer for software-assisted image analysis of

peptide microarray data

• GenePix® Pro support with GAL files

Immune Profiling of Blood Sera

• fingerprint analysis of immune responses in sera linked with infection, vaccination, oncogenesis, autoimmune diseases

• identification of differential signatures in patient and control groups • custom and off-the-shelf contents available covering various indications,

organisms, strains, subtypes and antigen collection

• high reproducibility and data consistency for statistical analysis of microarray data

• material saving with suited PEPperCHIP® Incubation Trays

PePperChiP

®

Applications

PePperMAP

®

Services

PEPperCHIP

®

Variants

Benefit from custom contents adjusted to your research:

• display your antigen(s) as overlapping peptides for epitope mapping • use more than 4,100 random peptides for mimotope discovery and

serum signaturing

• decode known peptides and epitopes with full permutation scans • translate custom peptide lists into peptide microarrays

• include multiple array copies per chip for one-by-one tests

• numerous standard contents available (antigens from EBV, HBV, H1N1, HCV, Dengue virus, HPV, melanoma etc.)

PEPperMAP

®

Elements

• a custom peptide microarray with your antigen as overlapping peptides

• all immunoassays with your antibody or serum sample and secondary antibodies

• control assays of Flag and HA control peptides • read-out of microarray data

• data quantification and evaluation

• detailed report with protocols, experimental details and results

Epitope Mapping

• characterization of mono- & polyclonal antibodies and sera

• proteins are translated into overlapping peptides with maximum peptide-peptide overlap

• custom peptide mapping contents from single to multiple antigens

• variable peptide lengths and overlap

• up to five one-by-one tests per chip possible • minimal sample consumption

PEPperCHIP® Incubation Tray (3/4 well format)

Antigen/Epitope/Mimotope Discovery

Access high content microarrays with up to 25,000 peptides for the discovery and development of:

• new epitopes and mimotopes

• serum biomarker and antibody signatures • active antigens in complex protein collections • protein target binders and affinity tags

• peptide hit discovery and lead optimization

PEPperMAP

®

Options

According to your demand, we offer various epitope mapping options from only € 675 on as well as completely customized PEPperMAP® solutions.

• Type 1 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 13mer peptides with a peptide-peptide overlap of 12 amino acids

• Type 2 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 10, 12 and 15mer peptides with a peptide-peptide overlap of 9, 11 and 14 amino acids

• Type 1+/2+ Mapping with success-related fees

• Type 3 Mapping: Full epitope scans with thorough sequence permutations

Please inquire or download our Product Catalog (www.pepperprint.com/ PEPperPRINT_Catalog.pdf) for a comprehensive product overview and pricing information.

High content PEPperCHIP® with 25,000 peptides for antigen, epitope, mimotope and target binder discovery (right)

Custom solutions from single antigens to full proteome sets.

Epitope mapping of a monoclonal antibody with a single epitope (left) compared to a polyclonal sample (bottom) with two epitopes.

Green/red frames: Staining of routinely implemented HA and Flag control peptides.

(3)

PePperChiP

®

PEPperCHIP

®

Specifications

• custom and off-the-shelf peptide microarrays • up to 9,000 features per chip

• thorough customization covering any protein or peptide collection

• fully adjusted to your research

• standard slide formats with wide chip reader compatibility

• GenePix® Pro and PepSlide® Analyzer support

• most cost effective solutions

PEPperMAP

®

Specifications

Benefit from PEPperPRINT’s state-of-the-art peptide microarray platform PEPperCHIP® without

any need for time, manpower or technical infra-structure.

Just send us your antibody or serum sample and let PEPperPRINT’s experts get the results for you. We are pleased to provide you with standard epitope mapping services and custom serum profiling solutions for your research.

PEPperCHIP® - PEPperPRINT’s new peptide microarray platform with custom and standard contents

PEPperMAP® - The most convenient way to benefit from PEPperPRINT’s new peptide microarray platform.

Custom peptide microarrays with more than 8,000 features for the discovery of serum signatures (left) and full permutation scans of a known antibody epitope (right)

Epitope mapping of a polyclonal antibody against two antigens as overlapping peptides in double spots (red; stained control peptides frame the antigen-derived peptides)

PEPperCHIP

®

Accessories

• PEPperCHIP® Incubation Trays with 3/1, 3/2, 3/3, 3/4, 3/5, 3/16 and 3/(3,4,5) well formats

3

3 one-by-one assays with multiple array copies

3

3 minimal sample consumption

3

3 enables shaking incubation

• PEPperCHIP® Staining Kit with labeled anti-HA and anti-Flag

antibodies for control assays

• PepSlide® Analyzer for software-assisted image analysis of

peptide microarray data

• GenePix® Pro support with GAL files

Immune Profiling of Blood Sera

• fingerprint analysis of immune responses in sera linked with infection, vaccination, oncogenesis, autoimmune diseases

• identification of differential signatures in patient and control groups • custom and off-the-shelf contents available covering various indications,

organisms, strains, subtypes and antigen collection

• high reproducibility and data consistency for statistical analysis of microarray data

• material saving with suited PEPperCHIP® Incubation Trays

PePperChiP

®

Applications

PePperMAP

®

Services

PEPperCHIP

®

Variants

Benefit from custom contents adjusted to your research:

• display your antigen(s) as overlapping peptides for epitope mapping • use more than 4,100 random peptides for mimotope discovery and

serum signaturing

• decode known peptides and epitopes with full permutation scans • translate custom peptide lists into peptide microarrays

• include multiple array copies per chip for one-by-one tests

• numerous standard contents available (antigens from EBV, HBV, H1N1, HCV, Dengue virus, HPV, melanoma etc.)

PEPperMAP

®

Elements

• a custom peptide microarray with your antigen as overlapping peptides

• all immunoassays with your antibody or serum sample and secondary antibodies

• control assays of Flag and HA control peptides • read-out of microarray data

• data quantification and evaluation

• detailed report with protocols, experimental details and results

Epitope Mapping

• characterization of mono- & polyclonal antibodies and sera

• proteins are translated into overlapping peptides with maximum peptide-peptide overlap

• custom peptide mapping contents from single to multiple antigens

• variable peptide lengths and overlap

• up to five one-by-one tests per chip possible • minimal sample consumption

PEPperCHIP® Incubation Tray (3/4 well format)

Antigen/Epitope/Mimotope Discovery

Access high content microarrays with up to 25,000 peptides for the discovery and development of:

• new epitopes and mimotopes

• serum biomarker and antibody signatures • active antigens in complex protein collections • protein target binders and affinity tags

• peptide hit discovery and lead optimization

PEPperMAP

®

Options

According to your demand, we offer various epitope mapping options from only € 675 on as well as completely customized PEPperMAP® solutions.

• Type 1 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 13mer peptides with a peptide-peptide overlap of 12 amino acids

• Type 2 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 10, 12 and 15mer peptides with a peptide-peptide overlap of 9, 11 and 14 amino acids

• Type 1+/2+ Mapping with success-related fees

• Type 3 Mapping: Full epitope scans with thorough sequence permutations

Please inquire or download our Product Catalog (www.pepperprint.com/ PEPperPRINT_Catalog.pdf) for a comprehensive product overview and pricing information.

High content PEPperCHIP® with 25,000 peptides for antigen, epitope, mimotope and target binder discovery (right)

Custom solutions from single antigens to full proteome sets.

Epitope mapping of a monoclonal antibody with a single epitope (left) compared to a polyclonal sample (bottom) with two epitopes.

Green/red frames: Staining of routinely implemented HA and Flag control peptides.

(4)

PePperChiP

®

PEPperCHIP

®

Specifications

• custom and off-the-shelf peptide microarrays • up to 9,000 features per chip

• thorough customization covering any protein or peptide collection

• fully adjusted to your research

• standard slide formats with wide chip reader compatibility

• GenePix® Pro and PepSlide® Analyzer support

• most cost effective solutions

PEPperMAP

®

Specifications

Benefit from PEPperPRINT’s state-of-the-art peptide microarray platform PEPperCHIP® without

any need for time, manpower or technical infra-structure.

Just send us your antibody or serum sample and let PEPperPRINT’s experts get the results for you. We are pleased to provide you with standard epitope mapping services and custom serum profiling solutions for your research.

PEPperCHIP® - PEPperPRINT’s new peptide microarray platform with custom and standard contents

PEPperMAP® - The most convenient way to benefit from PEPperPRINT’s new peptide microarray platform.

Custom peptide microarrays with more than 8,000 features for the discovery of serum signatures (left) and full permutation scans of a known antibody epitope (right)

Epitope mapping of a polyclonal antibody against two antigens as overlapping peptides in double spots (red; stained control peptides frame the antigen-derived peptides)

PEPperCHIP

®

Accessories

• PEPperCHIP® Incubation Trays with 3/1, 3/2, 3/3, 3/4, 3/5, 3/16 and 3/(3,4,5) well formats

3

3 one-by-one assays with multiple array copies

3

3 minimal sample consumption

3

3 enables shaking incubation

• PEPperCHIP® Staining Kit with labeled anti-HA and anti-Flag

antibodies for control assays

• PepSlide® Analyzer for software-assisted image analysis of

peptide microarray data

• GenePix® Pro support with GAL files

Immune Profiling of Blood Sera

• fingerprint analysis of immune responses in sera linked with infection, vaccination, oncogenesis, autoimmune diseases

• identification of differential signatures in patient and control groups • custom and off-the-shelf contents available covering various indications,

organisms, strains, subtypes and antigen collection

• high reproducibility and data consistency for statistical analysis of microarray data

• material saving with suited PEPperCHIP® Incubation Trays

PePperChiP

®

Applications

PePperMAP

®

Services

PEPperCHIP

®

Variants

Benefit from custom contents adjusted to your research:

• display your antigen(s) as overlapping peptides for epitope mapping • use more than 4,100 random peptides for mimotope discovery and

serum signaturing

• decode known peptides and epitopes with full permutation scans • translate custom peptide lists into peptide microarrays

• include multiple array copies per chip for one-by-one tests

• numerous standard contents available (antigens from EBV, HBV, H1N1, HCV, Dengue virus, HPV, melanoma etc.)

PEPperMAP

®

Elements

• a custom peptide microarray with your antigen as overlapping peptides

• all immunoassays with your antibody or serum sample and secondary antibodies

• control assays of Flag and HA control peptides • read-out of microarray data

• data quantification and evaluation

• detailed report with protocols, experimental details and results

Epitope Mapping

• characterization of mono- & polyclonal antibodies and sera

• proteins are translated into overlapping peptides with maximum peptide-peptide overlap

• custom peptide mapping contents from single to multiple antigens

• variable peptide lengths and overlap

• up to five one-by-one tests per chip possible • minimal sample consumption

PEPperCHIP® Incubation Tray (3/4 well format)

Antigen/Epitope/Mimotope Discovery

Access high content microarrays with up to 25,000 peptides for the discovery and development of:

• new epitopes and mimotopes

• serum biomarker and antibody signatures • active antigens in complex protein collections • protein target binders and affinity tags

• peptide hit discovery and lead optimization

PEPperMAP

®

Options

According to your demand, we offer various epitope mapping options from only € 675 on as well as completely customized PEPperMAP® solutions.

• Type 1 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 13mer peptides with a peptide-peptide overlap of 12 amino acids

• Type 2 Mapping: Epitope mapping of your antibody or serum sample against a protein translated into 10, 12 and 15mer peptides with a peptide-peptide overlap of 9, 11 and 14 amino acids

• Type 1+/2+ Mapping with success-related fees

• Type 3 Mapping: Full epitope scans with thorough sequence permutations

Please inquire or download our Product Catalog (www.pepperprint.com/ PEPperPRINT_Catalog.pdf) for a comprehensive product overview and pricing information.

High content PEPperCHIP® with 25,000 peptides for antigen, epitope, mimotope and target binder discovery (right)

Custom solutions from single antigens to full proteome sets.

Epitope mapping of a monoclonal antibody with a single epitope (left) compared to a polyclonal sample (bottom) with two epitopes.

Green/red frames: Staining of routinely implemented HA and Flag control peptides.

(5)

High Content Peptide Microarrays

Epitope Mapping • Serum Profiling • Peptide Engineering

PEPperCHIP

®

Get access to custom and standard peptide microarrays with up to 9,000 features per chip in a uniquely flexible and cost-effective manner.

PEPperMAP

®

Benefit from our new peptide microarray platform and let PEPperPRINT's experts get the results for you.

High Content Peptide Microarrays

Epitope Mapping • Serum Profiling • Peptide Engineering

PEPperCHIP

®

Get access to custom and standard peptide microarrays with up to 9,000 features per chip in a uniquely flexible and cost-effective manner.

PEPperMAP

®

Benefit from our new peptide microarray platform and let PEPperPRINT's experts get the results for you.

PEPperPRINT GmbH Rischerstraße 12 D-69123 Heidelberg Fon: +49 (0) 62 21 72 64 489 Fax: +49 (0) 62 21 72 64 475 pepperchip@pepperprint.com www.pepperprint.com

PePperChiP

®

High-Content Peptide Microarrays

PepSlide

®

Analyzer

Custom Peptide Synthesis

PePperMAP

®

Epitope Mapping & Serum Profiling Services

PepSlide

®

Analyzer Specifications

Quantify and analyze your peptide microarray data with our intuitive and easy-to-use software solution:

• use microarray scanners‘ TIFF images and load design data from GAL files or PepSlide® PSF files

• efficient tools for image processing, array alignment and spot finding

• perfectly adapted to the PEPperCHIP® platform

• full compatibility with Excel

• recognition of custom spot sizes and morphologies • state of the art background correction

• cost-effective alternative to GenePix® Pro

Peptide Specifications

• HPLC purified custom peptides (95% purity on demand)

• 1 mg (€ 11/amino acid), 5 mg (€ 22/amino acid) and 10 mg (€ 33/amino acid) batches

• 16, 96 and 384-well small scale peptide libraries • linear and cyclic peptides

• various modifications available (phosphorylation, PEGylation, biotinylation, acetylation, methylation etc.) • useful for competition and enzymatic assays, peptide

immobilization and peptide array spotting

Please inquire for other peptide purities, quantities and modifications.

(6)

High Content Peptide Microarrays

Epitope Mapping • Serum Profiling • Peptide Engineering

PEPperCHIP

®

Get access to custom and standard peptide microarrays with up to 9,000 features per chip in a uniquely flexible and cost-effective manner.

PEPperMAP

®

Benefit from our new peptide microarray platform and let PEPperPRINT's experts get the results for you.

High Content Peptide Microarrays

Epitope Mapping • Serum Profiling • Peptide Engineering

PEPperCHIP

®

Get access to custom and standard peptide microarrays with up to 9,000 features per chip in a uniquely flexible and cost-effective manner.

PEPperMAP

®

Benefit from our new peptide microarray platform and let PEPperPRINT's experts get the results for you.

PePperChiP

®

High-Content Peptide Microarrays

PepSlide

®

Analyzer

Custom Peptide Synthesis

PePperMAP

®

Epitope Mapping & Serum Profiling Services

PepSlide

®

Analyzer Specifications

Quantify and analyze your peptide microarray data with our intuitive and easy-to-use software solution:

• use microarray scanners‘ TIFF images and load design data from GAL files or PepSlide® PSF files

• efficient tools for image processing, array alignment and spot finding

• perfectly adapted to the PEPperCHIP® platform

• full compatibility with Excel

• recognition of custom spot sizes and morphologies • state of the art background correction

• cost-effective alternative to GenePix® Pro

Peptide Specifications

• HPLC purified custom peptides (95% purity on demand)

• 1 mg (€ 11/amino acid), 5 mg (€ 22/amino acid) and 10 mg (€ 33/amino acid) batches

• 16, 96 and 384-well small scale peptide libraries • linear and cyclic peptides

• various modifications available (phosphorylation, PEGylation, biotinylation, acetylation, methylation etc.) • useful for competition and enzymatic assays, peptide

immobilization and peptide array spotting

Please inquire for other peptide purities, quantities and modifications.

BioCat GmbH

Tel.: +49 6221 7141516

Email: info@biocat.com

Im Neuenheimer Feld 584

Fax: +49 6221 7141529

Web: www.biocat.com

D-69120 Heidelberg

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