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Degradation of Saccharomyces cerevisiae Transcription Factor Gcn4 Requires a C-Terminal Nuclear Localization Signal in the Cyclin Pcl5

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Figure

TABLE 1. Strains used in this study
TABLE 2. Plasmids used in this study
FIG. 1. Pcl5-GFP is transported into the S. cerevisiae nucleus.(A) Nuclear localization of high and low amounts of the functional
FIG. 2. Nuclear localization of yeast Pcl5 is required for Gcn4 degradation. (A) from the low-copy plasmid pME2848 alone ortogether withtohigh-copy plasmids under the control of thehigh-copy (2was analyzed by Western blotting with monoclonal anti-GFP antib
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