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Quantifying and Understanding Well-to-Well Contamination in Microbiome Research

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Figure

FIG 1 Plate design and experimental design. (a) NTC, sink, and source samples are distributed in a checkboardpattern across the plate
FIG 2 Example of plates with cross-contamination. Each panel depicts a 96-well plate, with source, sink, and blankwells denoted by “O,” “X,” and empty squares, respectively
FIG 3 Distance-decay relationship of source samples contaminating surrounding samples
FIG 4 Summary statistics of sample fraction compositions of well-to-well contaminants compared across extraction types(blanks [pink], sink [blue], and source [purple]) and across extraction methods (tube versus plate)
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