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Transgenically mediated shRNAs targeting conserved regions of foot-and-mouth disease virus provide heritable resistance in porcine cell lines and suckling mice

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Academic year: 2020

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Figure

Figure 1 Schematic diagram of the shRNA-expressing vectors used for transgenic modification.been substituted with non-specific shRNA cassettes
Table 1 The PCR primers used for detection and qPCR oftransgenic cells and mice
Figure 3 Establishment of transgenic monoclonal colonies andthe development of MTCL. The shRNA-expressing plasmids PB-EN3D2B or PB-ENlacZNH21 were integrated into the genome ofIBRS-2 cells using the piggyBac transposon system
Figure 4 Endogenous shRNA in MTCL conferred natural resistance to FMDV infection. Eight cell lines and normal IBRS-2 cells werechallenged with 20 TCID50 of FMDV O/HKN/2002 (A), 50 TCID50 of FMDV O/HKN/2002 (B) or 20 TCID50 of FMDV Asia 1/Jiangsu/2005 (C)
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