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High-level intracellular expression of heterologous proteins in Brevibacillus choshinensis SP3 under the control of a xylose inducible promoter

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Academic year: 2020

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Figure 1 Time course of intracellular GFP production byand GFP-pHis1522 after 48 h of growth at 37°C, 30°C, 25°C (about 20 ODGFP-pHis1522 grown at 37°C (red line), 30°C (green line), 25°C (blue line)30°C Brevibacillus choshinensis SP3 at different temperat
Figure 2 Time course of intracellularcarrying TcdA-GT-pNI and TcdA-GT-pHis1522 grown at 25°C(blue line)A α-amylase and TcdA-GT production by Brevibacillus choshinensis SP3 at different temperatures.) α-amylase activity of Brevibacillus lysate carrying α-am
Figure 3 Time course of extracellular GFP production by-pHis1522 grown at 25°C. SEC-GFP-pHis1522 was induced with 0,5% xylose
Figure 5 GFP, α-amylase, and TcdA-GT production yield in Brevibacillus SP3. Comparison of the maximum yield obtained for each protein/vector combination used in this study
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