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An Automated Perifusion System for Modifying Cell Culture Conditions over Time

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Figure

Fig. 1 Schematic of the automated syringe system used for experiments. Note that the collection beaker was replaced with a 96 well plate forthe trypan blue experiments (Figs
Fig. 2 Absorbance readings from the trypan blue (3 min on:3 min off) test. The syringe pump alternated between pumping 3 min of trypan bluethen 3 min of water for the entirety of the experiment
Fig. 4 Forced oscillations in islets. a Example of glucose-stimulated [Ca2+]i results from a forced oscillations experiment containing N = 9 isletsover a period of 55 min

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