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High-performance gene expression and knockout tools using sleeping beauty transposon system

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Academic year: 2020

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Figure

Fig. 1 Overview of the Sleeping Beauty (SB) transposon system. a The Gateway cloning of pSB vectors
Fig. 2 Overexpression of FBW7 using various vectors and identification of new associated protein of FBW7 though Tandem Affinity Purification.expressing either Flag-FBW7, Myc-FBW7, HA-FBW7 or V5-FBW7 are indicated
Fig. 3 FBW7 interacts with NFATc1 and promotes its Lys63-linked polyubiquitylation.using anti-IgG, anti-FBW7 or anti-NFATc1 as indicated, and were analyzed by Western blot according to standard procedures (n = 3)
Fig. 4 Depletion of RCC2 impairs mitotic entry and prometaphase to metaphase progression.(100 ng/ml) or Taxol (2histone H3 (pH 3) is indicated.0.05
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