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<p>Enhanced and Prolonged Antitumor Effect of Salinomycin-Loaded Gelatinase-Responsive Nanoparticles via Targeted Drug Delivery and Inhibition of Cervical Cancer Stem Cells</p>

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Figure 1 INIRF images. HeLa tumor bearing mice were systematically injected ofNIR-797 labeled Sal NPs, and observed for a period of 120h
Figure 2 In vivo antitumor efficacy of Sal and Sal NPs in a HeLa tumor model. (A) The tumor growth curves of HeLa tumor-bearing mice that received different treatments(n=8) ***p<0.001; (B) H&E staining of the tumors in different treatment groups (n=8); (C) Representative IHC staining of Ki-67, Caspase3 and PCNA of the cervicaltumors in day 12.Abbreviations: Sal, Salinomycin; Sal NPs, Sal-mPEG-pep-PCL nanoparticles; H&E, hematoxylin and eosin; IHC, Immunohistochemistry.
Figure 3 In vivo toxicity analysis of Sal and Sal NPs. (A): Histological analyses of organ toxicity through H&E staining (×200) after treatment with control, Blank NP, Sal free2, Sal NP 2, Sal NP 8; (B): Weight loss profiles over time after various treatments (n=8) ***p<0.001.Abbreviations: Sal, Salinomycin; Sal NPs, Sal-PEG-pep-PCL nanoparticles; H&E, hematoxylin and eosin; Sal free 2, salinomycin solvent 2 mg/kg, every other day for fourtimes; Sal NP 2, Sal NPs 2mg/Kg, every other day for 4 times; Sal NP 8, Sal NPs 8mg/kg, once.
Figure 4 Sal NPs inhibits stem like properties of CC in vivo. The IHC (A) and WB (B) and analysis of CD44 and CD133 variation in tumors of various treatment groups
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