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Developmental regulation of acidic fibroblast growth factor (aFGF) expression in bovine retina

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Academic year: 2020

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Figure

Fig. 1. aFGFin situ hybridizationon adultretina.Retina sectionsfrom7-year-oldbovine stainedwith hematoxylin-eosin(AI or hybndlzedwirhspecificaFGF antisenseriboprobe(B).(AIBrightfield(x180);(B!phasecontrast(x600)
Fig. 2. aFGF and opsin in situpresent hybridizationon developingretina.Retinasectionsfrom86-day-oldcaff embryo(AI, 5-month-oldcalf (8) and3-year-oldcow (C) werehybridizedwithaFGFanti sense(1,2)
Fig. 3. aFGF in situhybridizationfromembryoto old retina.Retinasectionsfrom 4-5-month(A), 8-9-month-IB)-ofdcalf embryosand 7-year-old bovine IC) were hybridizedwith a FGF antisense(1,2) or aFGF sense(control) (3)
Fig. 4. aFGF immunodetectionin 3-year-old bovine retina. IA.B) Anti-aFGF serum; IC) pre/mmuneserum; (D) phosphatebufferedsaline (PBS).(A) Phase contrast;(B, C, D) fluorescence.The immunohistochemistrysimplestaining procedurerevealsa diffuseintranuclearloca

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