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The role of platelet derived growth factor in the development of mouse molars

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Figure

Fig. ,. RT-PCR amplificationof PDGFA mRNA 1189 bp) isolatedfromenamelorganepithelia(e) or dentalmesenchyme1m) of E15 throughPN2 mousemandibularfirst molars.The enrire toorh germwas used !:om £14 embryo.The ilrst lane (marked M) is the 123 bp DNA ladder (GI
Fig. 3. Changesin thymidinecontentincorporation,protein,DNA and RNAin mousemandibularfirst and secondmolarexplantsfol-lowing exogenousPDGF-AAadministrationfor 7 days (7 dc-daysinculture)
Fig. 4. RT-PCRamplification of dental matrix protein transcriptsfromcentrationPDGF treatedmousemandibularfirstandsecondmolarex plantsIE15+7dc).Lanes M: the 123 bp DNA marker bands; lanes C:control; lanes .25: PDGF.AA at concentrationof 0.25 ng/m/; lanes 2.
Fig. 5. Changesin thymidineadministration.incorporationand proteincontentinrat pulpcell culturesfollowingexogenousPDGF.AA or PDGF-BBAsteriskdenotesstatistic significance at p<O.05.PDGFsat concentration of 25 ng/ml increased cell proliferation but nottotal protein accumulation in these cultured pulp cells.

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