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Human Coronavirus 229E Nonstructural Protein 13: Characterization of Duplex-Unwinding, Nucleoside Triphosphatase, and RNA 5′-Triphosphatase Activities

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TABLE 1. Analysis of HCoV-229E nsp13 NTPasesubstrate specificitya
FIG. 1. HCoV-229E nsp13 has RNA 5�panels A and B, the 5G-3cubated with HEL and HEL_KA, and the reaction products wereseparated by thin-layer chromatography (A) or polyacrylamide gelelectrophoresis (B) and visualized by autoradiography
FIG. 2. Inhibition of the HEL-associated RNA 5�activity by ATP. The plot illustrates the effect of including variousconcentrations of ATP, ADP, and AMP on the RNA 5activity of HEL

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