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Molecular Basis for Mitochondrial Localization of Viral Particles during Beet Necrotic Yellow Vein Virus Infection

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Figure

FIG. 1. Organization of the gene constructs used for the present study. (A) Plasmid pB2-14 contains the complete cDNA for BNYVV RNA-2in pBluescribe (4, 53)
FIG. 2. Expression of GFP-fused P75 deletion mutants in plant protoplasts. N. tabacumand the in vitro transcripts derived from constructs pB2-RT-GFP1 (panels A to C), pB2-RT-I), pRep-MTS-GFP (panels J to L), and pRep-MTS-IS-TM1-GFP (panels M to O)
FIG. 3. Expression of GFP fusions with different P75 domains in plant protoplasts. N. tabacumRNA-1 and the in vitro transcripts derived from constructs pRep-TM1-GFP (panels A to F), pRep-TM3-TM4-GFP (panels G to I), andpRep-MTS-IS-TM3-TM4-GFP (panels J to
FIG. 4. In vitro anchoring of full-length P75 to isolated plant mito-chondria. (A) Labeled P75 and CP were obtained by in vitro transcription/
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