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Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser

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Figure

Fig. 1. Schematic of the setup for two-photon microscopy. The frequency-doubled OPO signal (500–800 nm) and the residual pump beam (680–1080 nm) were filtered by a long-pass filter (LP)
Fig. 2. (A) The measured two-photon excitation spectrum for DAPI. The data points are indicated with diamonds and the lines are a guide to the eye.The error bars indicate the SD over the data set
Fig. 3. (A) Normalized intensity decays for DAPI-labelled cell nuclei in 3T3 cells excited at four different wavelengths
Fig. 5. Excitation spectra for (A) Hoechst 33258 with Pdiamonds and line) and AlexaFluor 350 (red crosses and line) with Pexc = 5 mW,(B) Calcofluor White with Pexc = 2.5 mW and (C) SYTOX Green (blackexc =10 mW.
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