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Fate of Infecting Simian Virus 40-Deoxyribonucleic Acid in Nonpermissive Cells: Integration into Host Deoxyribonucleic Acid

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Academic year: 2019

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FIG.1.padsplasticbyactivitykeptresuspen2dedtrichloroacetictrichloroaceticdextrantofculturescatedcountssalinievested3T3werewas pelletinig deoxyribontulclease Uptake of '4C-labeled SV40 DNAI into cells
FIG. 2.freshDNAcells0.2asturesofanidcover described. phosphate-buffered Relationiship betweeni uptake ofSV40 DNA I production of T-anitigeni
FIG. 4.fugedDNAscribeddescribedeitheror6wereCsCI-EtBrvelocity to purified Alkaline CsCl equilibrium centrifugation of isolated from infected and noninfected cells (a); S ,ug of 3H-cell DNA (0) extracted as described SV40 DNA I (0) was mixed with the soluti
FIG. 5.filterfromployed).bothcontrolbound14C-SV40byDNAdescribedneutralized,cRNA.centrifuged3T3describedtracted Detectioni of SV40 specific DNA sequences infected cells by hybridization with SV40 cRNA

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