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Transferrin-bearing polypropylenimine dendrimer for targeted gene delivery to the brain

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Figure

Figure 1. Epifluorescence microscopy imaging of the cellular uptake of Cy3- labeled DNA (2.5 µg/ well) complexed with DAB-Tf, after incubation for 15 min, 30 min, 45 min, 1 h, 2 h or 4 h with bEnd.3 cells (Blue: nuclei stained with DAPI (excitation: 405 nm
Figure 2. Epifluorescence microscopy imaging of the cellular uptake of Cy3- labeled DNA (2.5 µg/ well) either complexed with DAB-Tf, DAB or in solution, after incubation for 2 hours with bEnd.3 cells (Blue: nuclei stained with DAPI (excitation: 405 nm, laser line
Figure 3. Flow cytometry quantification of the cellular uptake fluorescein- labeled DNA (2.5 µg/ well) either complexed with DAB-Tf, DAB or in solution, after incubation for 2 hours with bEnd.3 cells (n=15) * : P <0.05 compared with DAB-Tf-DNA
Figure 4. Epifluorescence microscopy imaging of the bEnd.3 cellular uptake of Cy3- labeled DNA (2.5 µg/ well) complexed with DAB-Tf, following pre-treatment with various concentrations of free Tf (ranging from 2.5 µM to 20 µM)
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