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454 antibody sequencing error characterization and correction

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Figure

Table 1 454 sequencing of six different antibodies atdifferent concentrations (3-fold dilution) produceddifferent number of sequences (number in parenthesesdenotes the results from the second run)
Table 2 The consequence of 454 antibody sequencingerrors affecting the functionality of antibodies asdetermined by IMGT/HighV-QUEST using sequence dataobtained from the two repeat 454 runs, 1 and 2
Table 3 Insertion/deletion occurred in multiple reads mostly at homopolymeric regions of 2-3 nucleotide length aswell as at the random sites, for example, shown for run 1 of control antibody #1
Figure 3 (a), (b) The nucleotide composition analysis showing compositional differences in A, G, T and C (dA, dG, dT and dC) resultingfrom insertion, deletion or substitution or by combination as observed in erroneous sequence reads, respectively, from Run

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