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Mutations in Classical Swine Fever Virus NS4B Affect Virulence in Swine

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Figure

FIG. 1. (A) Alignment of the CSFV NS4B TIR domain against Sus scrofa(hTLR1). Shown are amino acid sequences between positions 195 and 204 (box 1) and between positions 228 and 235 (box 2) of the CSFV NS4Bprotein
FIG. 2. (A) Schematic representation of substitutions at box 1 and box 2 of classical swine fever virus NS4B protein, generated by site-directedmutagenesis of the full-length cDNA clone pBIC
TABLE 1. Swine survival and fever response following infectionwith CSFV mutant NS4B.VGIv or the parental strain, BICv
TABLE 3. Titers of virus in tissues after intranasal inoculation with mutant NS4B.VGIv or the parental strain, BICv
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