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Cellular Topoisomerase I Modulates Origin Binding by Bovine Papillomavirus Type 1 E1

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Figure

FIG. 1. Topo I stimulates the origin binding activity of E1. (A) Afixed amount of E1 (0.5 pmol) and increasing amounts of Topo I (0 to
FIG. 2. Topo I stimulation of E1 binding is highly specific. (A) Topo I does not stimulate the non-origin binding activity of E1
FIG. 3. The effects of ATP/Mg2(A) Increasing amounts of E1 (0.2, 0.5, and 1 pmol) were incubated with radiolabeled BPV1 origin DNA at 37°C or 25°C in the presence andabsence of 4 mM ATP/7 mM Mg50 fmol) were performed at 37°C or 25°C in the presence or abse
FIG. 5. Topo I is not present in the E1-DNA complex. The stim-ulation of E1 origin binding by Topo I was analyzed following addition
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