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Identification of a Novel Human Immunodeficiency Virus Type 1 Integrase Interactor, Gemin2, That Facilitates Efficient Viral cDNA Synthesis In Vivo

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Figure

FIG. 1. Interaction of IN with Gemin2. (A) Yeast AH109 or HF7ccells were cotransformed with the pGAD-IBDG2 vector carrying the
FIG. 2. Gemin2 interacts with the incoming HIV-1 genome com-plex. (A) Virus particles in the culture supernatant of 293T cells co-
FIG. 3. Involvement of cellular Gemin2 in HIV-1 replication. (A) Total HeLa cell lysates prepared 48 h after transfection of siGemin2(HeLa/siGemin2) or control siGFP (HeLa/siCont) were serially diluted twofold followed by Western blot analysis with antibodies against Gemin2,
FIG. 4. Effect of siGemin2 on HIV-1 infection and cDNA synthesisin primary MDMs. (A) MDMs were transfected with siGemin2 or
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