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Micro pharmacokinetics: quantifying local drug concentration at live cell membranes

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Figure

Figure 1. FCS experimental set-up used in this study. (a) Confocal image of receptor expressing (cell 1) and non-receptor expressing (cell 2) CHO-β2GFP cells, and control areas of no cell (area 3) used for FCS measurements
Figure 2. Role of the presence of the target receptor and the cell membrane on local BY-propranolol cells in the presence of ICI 118,551 at an equivalent distance from the coverslip (2-way ANOVA, Tukey’s post hoc test), whilst distances above the coverslip
Figure 3. Effect of incubation time on BY-propranolol concentrations in the immediate vicinity of cell incubation
Figure 4. Schematic representation of increased local ligand concentrations in the immediate vicinity of the cell membrane and target receptors compared to the bulk aqueous phase

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